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Recombinant Human Endostatin Protein

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SDS-Page: Recombinant Human Endostatin Protein [NBP2-34974]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Human Endostatin Protein Summary

Description
A single non-glycosylated polypeptide chain containing 183 amino acids corresponding to the C-terminal fragment of Endostatin Source: E. coli

Uniprot ID: P39060

Amino Acid Sequence: HSHRDFQPVL HLVALNSPLS GGMRGIRGAD FQCFQQARAV GLAGTFRAFL SSRLQDLYSI VRRADRAAVP IVNLKDELLF PSWEALFSGS EGPLKPGARI FSFDGKDVLR HPTWPQKSVW HGSDPNGRRL TESYCETWRT EAPSATGQAS SLLGGRLLGQ SAASCHHAYI VLCIENSFMT ASK

Details of Functionality
Endostatin protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2.0 ug/mL in the presence of Anti-Human Endostatin Polyclonal Antibody.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
COL18A1
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of Endostatin as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
20.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human Endostatin Protein

  • antiangiogenic agent
  • COL18A1
  • collagen alpha-1(XVIII) chain
  • collagen, type XVIII, alpha 1
  • Endostatin
  • FLJ27325
  • FLJ34914
  • human type XVIII collagen10endostatin
  • KNO
  • KNO1MGC74745
  • Knobloch syndrome, type 1
  • KS
  • multi-functional protein MFP

Background

Endostatin is a proteolytic fragment of type XVIII collagen. It inhibits endothelial cell growth, angiogenesis, and wound healing. It also induces the production of the vasorelaxant nitric oxide by endothelial and smooth muscle cells. These actions contribute to the ability of endostatin to block the progression of a wide variety of tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol COL18A1
Uniprot