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Endostatin Antibody [Unconjugated]

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Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lacteals. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF570
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF570-SP
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Endostatin Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Endostatin
His1105-Lys1288
Accession # AAA19787
Specificity
Detects mouse Endostatin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endostatin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COL18A1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF570 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endostatin Antibody [Unconjugated]

  • antiangiogenic agent
  • COL18A1
  • collagen alpha-1(XVIII) chain
  • collagen, type XVIII, alpha 1
  • Endostatin
  • FLJ27325
  • FLJ34914
  • human type XVIII collagen10endostatin
  • KNO
  • KNO1MGC74745
  • Knobloch syndrome, type 1
  • KS
  • multi-functional protein MFP

Background

Endostatin is a fragment of collagen XVIII and functions as an angiogenesis antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endostatin Antibody (AF570)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 5 applications: ELISA Development, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC
(2)
IHC-Fr
(1)
Western Blot
(2)
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Human
(1)
Mouse
(4)
Porcine
(1)
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Showing Publications 1 - 8 of 8.
Publications using AF570 Applications Species
Mark Hepokoski, Joshua A. Englert, Rebecca M. Baron, Laura E. Crotty-Alexander, Mark M. Fuster, Jeremy R. Beitler et al. Ventilator-induced lung injury increases expression of endothelial inflammatory mediators in the kidney American Journal of Physiology-Renal Physiology 4/1/2017 [PMID: 28365585]
Oliveira-Ferrer L, Wellbrock J, Bartsch U, Penas E, Hauschild J, Klokow M, Bokemeyer C, Fiedler W, Schuch G Combination therapy targeting integrins reduces glioblastoma tumor growth through antiangiogenic and direct antitumor activity and leads to activation of the pro-proliferative prolactin pathway. Mol Cancer, 2013-11-20;12(1):144. 2013-11-20 [PMID: 24257371] (Western Blot, Porcine) Western Blot Porcine
Michael B. Duncan, Changqing Yang, Harikrishna Tanjore, Patrick M. Boyle, Doruk Keskin, Hikaru Sugimoto et al. Type XVIII collagen is essential for survival during acute liver injury in mice Disease Models & Mechanisms 4/13/2013 [PMID: 23580202]
Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop. BMC Cancer, 2013-03-26;13(0):154. 2013-03-26 [PMID: 23530721] (ICC, IHC-Fr, Human) ICC, IHC-Fr Human
Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production. Br. J. Cancer, 2011-10-20;105(10):1615-24. 2011-10-20 [PMID: 22015555] (Western Blot, Mouse) Western Blot Mouse
Lan KL, Ou-Yang F, Yen SH, Shih HL, Lan KH Cationic liposome coupled endostatin gene for treatment of peritoneal colon cancer. Clin. Exp. Metastasis, 2010-04-07;27(5):307-18. 2010-04-07 [PMID: 20373131] (ELISA Development, Mouse) ELISA Development Mouse
Tanabe K, Maeshima Y, Ichinose K, Kitayama H, Takazawa Y, Hirokoshi K, Kinomura M, Sugiyama H, Makino H Endostatin peptide, an inhibitor of angiogenesis, prevents the progression of peritoneal sclerosis in a mouse experimental model. Kidney Int., 2006-12-27;71(3):227-38. 2006-12-27 [PMID: 17191085] (IHC, Mouse) IHC Mouse
Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells. Am. J. Pathol., 2006-11-01;169(5):1767-83. 2006-11-01 [PMID: 17071599] (IHC, Mouse) IHC Mouse

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Bioinformatics

Gene Symbol COL18A1
Uniprot