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EMP/MAEA Antibody

Images

 
Western Blot: MAEA Antibody [NBP2-17200] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: MAEA Antibody [NBP2-17200] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using MAEA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EMP/MAEA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EMP/MAEA. The exact sequence is proprietary.
Localization
Nucleus matrix; Cell membrane; Cytoplasm; cytoskeleton
Predicted Species
Mouse (98%), Rat (98%), Chicken (95%), Xenopus (95%), Zebrafish (93%), Rhesus Macaque (100%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAEA
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for EMP/MAEA Antibody

  • EMLP
  • EMP
  • EMPCell proliferation-inducing gene 5 protein
  • Erythroblast macrophage protein
  • HLC-10
  • Human lung cancer oncogene 10 protein
  • lung cancer-related protein 10
  • macrophage erythroblast attacher
  • MAEA
  • PIG5
  • proliferation-inducing gene 5

Background

This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAEA
Uniprot