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ELKS Antibody

Images

 
Independent Antibodies: Western Blot: ELKS Antibody [NBP1-88177] - Analysis using Anti-ERC1 antibody NBP1-88177 (A) shows similar pattern to independent antibody NBP1-88179 (B).
Immunocytochemistry/ Immunofluorescence: ELKS Antibody [NBP1-88177] - Staining of human cell line U-251 MG shows positivity in cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ELKS Antibody [NBP1-88177] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Western Blot: ELKS Antibody [NBP1-88177] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

ELKS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EEREEEMKQMEVYRSHSKFMKNKVEQLKEELSSKEAQWEELKKKAAGLQAEIGQVKQELSRKDTELLALQTKLETLINQFSD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ELKS Protein (NBP1-88177PEP)
Publications
Read Publication using
NBP1-88177 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ELKS Antibody

  • ELKS/RAB6-interacting/CAST family member 1
  • ELKSCAST2
  • ERC-1
  • FLJ31750
  • KIAA1081Cast2
  • MGC12974
  • RAB6 interacting protein 2
  • Rab6-interacting protein 2
  • RAB6IP2

Background

Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. The protein is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13). Five transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ELKS Antibody (NBP1-88177)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ERC1