![Western Blot: PAF49 Antibody [NBP2-19676] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PAF49-Antibody-Western-Blot-NBP2-19676-img0004.jpg "Western Blot: PAF49 Antibody [NBP2-19676] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary. |
| Localization | Nucleus; nucleolus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CD3EAP |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PAF49-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19676-img0002.jpg "Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: PAF49 Antibody [NBP2-19676] - Paraffin-embedded human ovarian cancer. PAF49 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PAF49-Antibody-Immunohistochemistry-Paraffin-NBP2-19676-img0003.jpg "Immunohistochemistry-Paraffin: PAF49 Antibody [NBP2-19676] - Paraffin-embedded human ovarian cancer. PAF49 antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/PAF49-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19676-img0001.jpg "Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.")
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![Immunohistochemistry Uromodulin Antibody [Unconjugated]](https://images.novusbio.com/images2/Uromodulin_AF5175_Immunohistochemistry_12099.jpg)
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![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")