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EGF Antibody (10827) [Unconjugated]

 (7 Publications)
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EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications IHC, ELISA(Cap)
Clone
10827
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB636-SP
Carrier Free
MAB636-500
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EGF Antibody (10827) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human EGF
Specificity
Detects human and primate EGF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑ alpha , hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha , or rhTNF-beta .
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EGF
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
Application Notes
ELISA Detection: Human EGF Biotinylated Antibody (Catalog number BAF236)
Standard: Recombinant Human EGF (Catalog number 236-EG)
Publications
Read Publications using
MAB636 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGF Antibody (10827) [Unconjugated]

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGF Antibody (MAB636)(7)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 3 applications: ELISA Capture, ELISA Development, Western Blot.


Filter By Application
ELISA Capture
(2)
ELISA Development
(2)
Western Blot
(1)
All Applications
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Human
(2)
Bovine
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB636 Applications Species
Tanida, T;Tagami, T;Sato, H;Kyaw, HM;Fujikawa, T;Nagano, M;Momozawa, K;Yanagawa, Y;Katagiri, S; Effects of recombinant osteopontin expressed in Escherichia coli on the recovery of the endometrial epidermal growth factor profile and fertility in repeat breeder dairy cows Theriogenology 2024-01-11 [PMID: 38280277] (ELISA Capture, Bovine) ELISA Capture Bovine
Phong, V;Fujikawa, T;Kawano, K;Yanagawa, Y;Katagiri, S; Effects of prostaglandin F2? treatment at follicular wave emergence on endometrial epidermal growth factor concentration on day 3 of the next estrous cycle and on fertility in dairy cows Theriogenology 2023-09-20 [PMID: 37748361] (ELISA Capture, Bovine) ELISA Capture Bovine
Nattapong NINPETCH, Dagvajamts BADRAKH, HAY MAR KYAW, Kohei KAWANO, Yojiro YANAGAWA, Masashi NAGANO et al. Leptin receptor expression and its change in association with the normalization of EGF profile after seminal plasma treatment in repeat breeder dairy cows Journal of Reproduction and Development 6/1/2022 [PMID: 35228409]
Kohei KAWANO, Yojiro YANAGAWA, Masashi NAGANO, Seiji KATAGIRI Effects of heat stress on the endometrial epidermal growth factor profile and fertility in dairy cows Journal of Reproduction and Development 4/1/2022 [PMID: 35095040]
Frank Götschel, Daniela Berg, Wolfgang Gruber, Christian Bender, Markus Eberl, Myriam Friedel et al. Synergism between Hedgehog-GLI and EGFR Signaling in Hedgehog-Responsive Human Medulloblastoma Cells Induces Downregulation of Canonical Hedgehog-Target Genes and Stabilized Expression of GLI1 PLoS ONE 6/10/2013 [PMID: 23762360]
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012-02-17;378(1):72-80. 2012-02-17 [PMID: 22366959] (ELISA Development, Human) ELISA Development Human
Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light. J. Biotechnol., 2006-04-03;125(2):159-72. 2006-04-03 [PMID: 16584796] (ELISA Development, Western Blot, Human) ELISA Development, Western Blot Human

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Isotype Controls

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Bioinformatics

Gene Symbol EGF