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EGF Antibody (10825) [Unconjugated]

 (15 Publications)
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Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more
EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse ...read more
Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clone
10825
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB236-SP
Carrier Free
MAB236-500
100% Guarantee on every product

EGF Antibody (10825) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human EGF
Specificity
Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EGF
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.05-0.1 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB236 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGF Antibody (10825) [Unconjugated]

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EGF Antibody
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EGF Antibody (10825) [Unconjugated]
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Publications for EGF Antibody (MAB236)(15)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 6 applications: Control, Functional Assay, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
Control
(1)
Functional Assay
(1)
IHC
(2)
IHC-P
(1)
Neutralization
(5)
Western Blot
(3)
All Applications
Filter By Species
Human
(11)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB236 Applications Species
Tamara Muliaditan, Leena Halim, Lynsey M. Whilding, Benjamin Draper, Daniela Y. Achkova, Fahima Kausar et al. Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors Cell Reports Medicine 12/21/2021 [PMID: 35028604]
E Ticiani, J Gingrich, Y Pu, M Vettathu, J Davis, D Martin, MG Petroff, A Veiga-Lope Bisphenol S and Epidermal Growth Factor Receptor Signaling in Human Placental Cytotrophoblasts Environmental health perspectives, 2021-02-19;129(2):27005. 2021-02-19 [PMID: 33605785] (Control, Human) Control Human
Ethan L. Morgan, James A. Scarth, Molly R. Patterson, Christopher W. Wasson, Georgia C. Hemingway, Diego Barba-Moreno et al. E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer Cell Death & Differentiation 5/1/2021 [PMID: 33303976]
Antonios Chronopoulos, Stephen D. Thorpe, Ernesto Cortes, Dariusz Lachowski, Alistair J. Rice, Vasyl V. Mykuliak et al. Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway Nature Materials 6/1/2020 [PMID: 31907416]
H Nagano, C Tomida, N Yamagishi, S Teshima-Ko VEGFR-1 Regulates EGF-R to Promote Proliferation in Colon Cancer Cells Int J Mol Sci, 2019-11-09;20(22):. 2019-11-09 [PMID: 31717527] (Neutralization, Human) Neutralization Human
S Jeong, B Kim, HC Lau, A Kim Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions Pharmaceutics, 2019-10-14;11(10):. 2019-10-14 [PMID: 31614977] (Western Blot, Human) Western Blot Human
S Müller-Deu, L Seefried, M Krug, F Jakob, R Ebert Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells Stem Cell Res, 2017-08-18;24(0):69-76. 2017-08-18 [PMID: 28843157] (Functional Assay, Human) Functional Assay Human
MB Arnli, T Backer-Grø, B Ytterhus, US Granli, S Lydersen, S Gulati, SH Torp Expression and clinical value of EGFR in human meningiomas PeerJ, 2017-03-29;5(0):e3140. 2017-03-29 [PMID: 28367377] (IHC, Human) IHC Human
Y Iwata Case report of multiple pustules of the bilateral lower limbs caused by a granulocyte colony-stimulating factor-producing solid pseudopapillary tumour of the pancreas Br. J. Dermatol, 2017-08-20;0(0):. 2017-08-20 [PMID: 27925156] (IHC, Human) IHC Human
Zhang Z, Dong Z, Lauxen I, Filho M, Nor J Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype. Cancer Res, 2014-03-31;74(10):2869-81. 2014-03-31 [PMID: 24686166] (Neutralization, Human) Neutralization Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol EGF