![Knockdown Validated: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Knockdown-Validated-NB110-61576-img0006.jpg "Knockdown Validated: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Po, CaSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Synthetic peptide made to an internal portion of human DUOX2 (within residues 400-500). [Swiss-Prot# Q9NRD8] |
| Localization | Apical cell membrane; Multi-pass membrane protein. Note: Localizes to the apical membrane of epithelial cells. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DUOX2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Western blot a band is observed at ~150 kDa, and in Immunocytochemistry/Immunofluorescence membrane staining was seen in A431 cells. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DUOX2 Antibody (NB110-61576)Find related products by research area.
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Toll-like receptors in the intestinal epithelial cells By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ... Read full blog post. |
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| Gene Symbol | DUOX2 |
![Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0002.jpg "Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.")
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0004.jpg "Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 in parental HeLa cells and three clones of NOX4 knockout cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170327), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0005.jpg "Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 in parental HeLa cells and three clones of NOX4 knockout cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170327), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0003.jpg "Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")