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SCGF/CLEC11a Antibody (239029) [Unconjugated]

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SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) overnight at 4 °C. Tissue was stained with ...read more
SCGF/CLEC11a was detected in immersion fixed IFN gamma activated THP-1 human acute monocytic leukemia cell line using 10 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) for 3 hours at room ...read more
Immunohistochemistry of SCGF expression in GIST samples. A) Hematoxylin-eosin section of one representative slide from GIST 5. B) SCGF staining; strong SCGF positivity, consistent with western blotting, was restricted ...read more
Western blotting of SCGF expression. Western blotting with anti SCGF-alpha /-beta antibody of tumor tissue samples from the untreated GIST 1 patient (lane A), the SCGF-positive GIST 5 patient (lane B), a pool of plasma ...read more
Analysis of an independent set of imatinib-treated GIST samples. A) Comparative analysis of tumoral areas from of GIST 6, 7 and 8, which resulted non-responding or responding to imatinib treatment. Panels 1-5, 6-10 and ...read more
Analysis of an independent set of imatinib-treated GIST samples. A) Comparative analysis of tumoral areas from of GIST 6, 7 and 8, which resulted non-responding or responding to imatinib treatment. Panels 1-5, 6-10 and ...read more
Immunohistochemistry of CD68 and SCGF in GIST 5 and GIST 2. A) Hematoxilin-eosin section of one representative slide from GIST 5. B) CD68 staining; CD68 positive macrophages are scattered through the section. C) SCGF ...read more
Immunohistochemistry of CD68 and SCGF in GIST 5 and GIST 2. A) Hematoxilin-eosin section of one representative slide from GIST 5. B) CD68 staining; CD68 positive macrophages are scattered through the section. C) SCGF ...read more
Immunohistochemistry of SCGF expression in GIST samples. A) Hematoxylin-eosin section of one representative slide from GIST 5. B) SCGF staining; strong SCGF positivity, consistent with western blotting, was restricted ...read more
Western blotting of SCGF after deglycosylation. Western blotting with anti-SCGF-alpha /-beta antibody. Lanes 1 and 2 compare SCGF patterns in the untreated (lane 1) and the deglycosylated (lane 2) protein extract of ...read more
Western blotting of SCGF after deglycosylation. Western blotting with anti-SCGF-alpha /-beta antibody. Lanes 1 and 2 compare SCGF patterns in the untreated (lane 1) and the deglycosylated (lane 2) protein extract of ...read more
Analysis of an independent set of imatinib-treated GIST samples. A) Comparative analysis of tumoral areas from of GIST 6, 7 and 8, which resulted non-responding or responding to imatinib treatment. Panels 1-5, 6-10 and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
239029
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1904-SP
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SCGF/CLEC11a Antibody (239029) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SCGF/CLEC11a
Gly19-Phe245
Accession # BAA21499
Specificity
Detects human SCGF/CLEC11a in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse SCGF-beta is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CLEC11A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1904 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCGF/CLEC11a Antibody (239029) [Unconjugated]

  • CLEC11a
  • CLECSF3
  • LSLCL
  • P47
  • SCGF

Background

Stem Cell Growth Factor (SCGF) is a secreted sulfated glycoprotein that belongs to the C-type lectin superfamily (CLEC11a) and is expressed primarily in hematopoietic tissues. It functions as a growth factor for primitive hematopoietic progenitor cells. SCGF-beta is a truncated variant of SCGF-alpha that has aa 78 amino acid deletion and lacks a functional calcium-dependent carbohydrate-recognition domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SCGF/CLEC11a
Antibody (239029) [Un ...
MAB1904
SCGF/CLEC11a Antibody (239029) [Unconjugated]
Species: Hu
Applications: WB, IHC, ICC/IF

Publications for SCGF/CLEC11a Antibody (MAB1904)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, Western Blot.


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Bioinformatics

Gene Symbol CLEC11A
Uniprot