![Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.](http://images.novusbio.com/fullsize/Cytochrome-b245-alpha-Antibody-CS9-Flow-Cytometry-NBP1-40974-img0003.jpg "Flow Cytometry: Cytochrome b245 alpha Antibody (CS9) [NBP1-40974] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain: FITC. Permeabilised with Leucoperm.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, IP |
| Clone | CS9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Cytochrome B245 solubilised in octyl-beta-gluycopyranoside |
| Specificity | This antibody recognises the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYBA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Membrane permeabilisation is required for Flow Cytometry. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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| Publication using NBP1-40974 | Applications | Species |
|---|---|---|
| Aurelius J, Hallner A, Werlenius O et al. NOX2-dependent immunosuppression in chronic myelomonocytic leukemia. J. Leukoc. Biol. 2017-03-14 [PMID: 28292946] (Human) | Human |
Secondary Antibodies |
Isotype Controls |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")