NOX1 Antibody Summary
Immunogen Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.
Localization Membrane, Multi-pass membrane protein
Isotype IgG
Clonality Polyclonal
Host Rabbit
Gene NOX1
Purity Immunogen affinity purified
Innovator's Reward Test in a species/application not listed above to receive a full credit towards a future purchase.
Applications/Dilutions
Dilutions
Flow Cytometry Validated from a verified customer review. Immunocytochemistry/ Immunofluorescence 1:100-1:1000 Immunohistochemistry 1:100-1:1000 Immunohistochemistry-Paraffin 1:100-1:1000 Immunoprecipitation Reported in scientific literature (PMID: 27094494) Western Blot 1:500-1:3000
Theoretical MW 65 kDa.
Reviewed Applications
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Publications
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Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).
Packaging, Storage & Formulations
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer PBS (pH 7), 20% Glycerol
Preservative 0.025% Proclin 300
Purity Immunogen affinity purified
Alternate Names for NOX1 Antibody
Background
The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for NOX1 Antibody (NBP1-31546)(34)
We have publications tested in 3 confirmed species: Human, Mouse, Rat.
We have publications tested in 7 applications: B/N, FLOW, ICC/IF, IHC-P, IHC-P WB, IP, WB.
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10 of 34.
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Publications using NBP1-31546
Applications
Species
Scharmacher J, Wartenberg M, Sauer H The pro-inflammatory signature of lipopolysaccharide in spontaneous contracting embryoid bodies differentiated from mouse embryonic stem cells Journal of cellular and molecular medicine 2023-06-14 [PMID: 37315183] (WB, Mouse) WB
Mouse
Yao G, Li H, Zuo X et al. Oscillatory shear stress promotes vein graft intimal hyperplasia via NADPH oxidase-related pathways Frontiers in Surgery 2023-02-13 [PMID: 36860953] (B/N, WB) B/N, WB
Hwang S, Kim SH, Yoo KH et al. Exogenous 8-hydroxydeoxyguanosine attenuates doxorubicin-induced cardiotoxicity by decreasing pyroptosis in H9c2 cardiomyocytes BMC molecular and cell biology 2022-12-14 [PMID: 36517746]
Meister ML, Najjar RS, Danh JP et al. Berry consumption mitigates the hypertensive effects of a high-fat, high-sucrose diet via attenuation of renal and aortic AT1R expression resulting in improved endothelium-derived NO bioavailability The Journal of nutritional biochemistry 2022-11-23 [PMID: 36435288] (WB, Mouse, Human) WB
Mouse, Human
Najjar R, Mu S, Feresin R Blueberry Polyphenols Increase Nitric Oxide and Attenuate Angiotensin II-Induced Oxidative Stress and Inflammatory Signaling in Human Aortic Endothelial Cells Antioxidants 2022-03-23 [PMID: 35453301] (WB, Human) WB
Human
Tarafdar A, Wolska N, Krisp C et al. The amyloid peptide beta disrupts intercellular junctions and increases endothelial permeability in a NADPH oxidase 1-dependent manner Redox Biology 2022-06-01 [PMID: 35358850] (WB, Human) WB
Human
Oh S, Yang JY, Park CH Et al. Dieckol Reduces Muscle Atrophy by Modulating Angiotensin Type II Type 1 Receptor and NADPH Oxidase in Spontaneously Hypertensive Rats Antioxidants (Basel, Switzerland) 2021-09-30 [PMID: 34679696] (WB, Rat) WB
Rat
Gaspar R, Sage T, Little G et al. Protein Disulphide Isomerase and NADPH Oxidase 1 Cooperate to Control Platelet Function and Are Associated with Cardiometabolic Disease Risk Factors Antioxidants 2021-03-23 [PMID: 33806982] (ICC/IF, Human) ICC/IF
Human
Gaspar RS, Ferreira PM, Mitchell JL et al. Platelet-derived extracellular vesicles express NADPH oxidase-1 (Nox-1), generate superoxide and modulate platelet function Free Radical Biology and Medicine 2021-03-01 [PMID: 33548451] (FLOW) FLOW
Pankaj Attri, Jae-Hyun Park, Joey De Backer, Myeongkyu Kim, Ji-Hye Yun, Yunseok Heo, Sylvia Dewilde, Masaharu Shiratani, Eun Ha Choi, Weontae Lee, Annemie Bogaerts Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study. International journal of biological macromolecules 2021-04-01 [PMID: 32961197]
Kumar Raut P, Park P Globular adiponectin antagonizes leptin-induced growth of cancer cells by modulating inflammasomes activation: Critical role of HO-1 signaling Biochemical Pharmacology 2020-07-01 [PMID: 32745467]
Wang X, Zhang S, Ding Y et al. p47phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis Redox Biol 2020-05-11 [PMID: 32422541] (WB, Mouse) WB
Mouse
Yoshimura Y Resveratrol slows cell growth by targeting the Warburg effect and stimulating mitochondria metabolism Thesis 2019-06-28 [PMID: 31304204] (WB, Human) WB
Human
Hong G, Davies C, Omole Z et al. Campylobacter jejuni modulates reactive oxygen species production and NADPH oxidase 1 expression in human intestinal epithelial cells bioRxiv 2022-03-07 (WB, Human)
WB
Human
Wang C, Zhu L, Yuan W et al. Diabetes aggravates myocardial ischaemia reperfusion injury via activating Nox2-related programmed cell death in an AMPK-dependent manner J. Cell. Mol. Med. 2020-04-29 [PMID: 32351005] (WB, Rat) WB
Rat
Cao L, Li X, Xu R et al. DUOX2, a common modulator in preventive effects of monoamine-based antidepressants on water immersion restraint stress- and indomethacin- induced gastric mucosal damage European Journal of Pharmacology 2020-03-01 [PMID: 32131022] (WB, Rat) WB
Rat
Shin SK, Kim KO, Kim SH et al. Exogenous 8-hydroxydeoxyguanosine ameliorates liver fibrosis through the inhibition of Rac1-NADPH oxidase signaling J. Gastroenterol. Hepatol. 2020-01-06 [PMID: 31907970] (IHC-P WB, Human, Rat) IHC-P WB
Human, Rat
Diebold BA, Wilder SG, De Deken X et al. Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR Methods Mol. Biol. 2019-06-08 [PMID: 31172474]
Kaushik DK, Bhattacharya A, Mirzaei R et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance J. Clin. Invest. 2019-05-13 [PMID: 31081803] (WB, Human) WB
Human
Vara D, Cifuentes-Pagano E, Pagano PJ, Pula G. A novel combinatorial technique for simultaneous quantification of oxygen radicals and aggregation reveals unexpected redox patterns in the activation of platelets by different physiopathological stimuli Haematologica 2019-01-24 [PMID: 30679320] (IP, Mouse, Human) IP
Mouse, Human
Raut PK, Kim SH, Choi DY et al. Growth of breast cancer cells by leptin is mediated via activation of the inflammasome: Critical roles of estrogen receptor signaling and reactive oxygen species production. Biochem. Pharmacol. 2019-01-10 [PMID: 30633869] (WB, Human) WB
Human
Tian HP, Sun YH, He L et al. Single-Stranded DNA-Binding Protein 1 Abrogates Cardiac Fibroblast Proliferation and Collagen Expression Induced by Angiotensin II. Int Heart J. 2018-10-25 [PMID: 30369577]
Stuart JA, Fonseca J, Moradi F et al. How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Oxid Med Cell Longev. 2018-09-30 [PMID: 30363917] (WB, Mouse, Human) WB
Mouse, Human
Shin SK, Cho JH, Kim EJ et al. Anti-inflammatory and anti-apoptotic effects of rosuvastatin by regulation of oxidative stress in a dextran sulfate sodium-induced colitis model World J. Gastroenterol. 2017-07-07 [PMID: 28740344] (ICC/IF, Mouse) ICC/IF
Mouse
Kangawa Y, Yoshida T, Maruyama K et al. Cilostazol and enzymatically modified isoquercitrin attenuate experimental colitis and colon cancer in mice by inhibiting cell proliferation and inflammation. Food Chem. Toxicol. 2016-12-15 [PMID: 27989839] (Mouse) Mouse
Wyssenbach A, Quintela T, Llavero F et al. Amyloid B-induced astrogliosis is mediated by B1-integrin via NADPH oxidase 2 in Alzheimer's disease. Aging Cell 2016-10-05 [PMID: 27709751] (IHC-P, Mouse) IHC-P
Mouse
Pekarcikova L, Knopfova L, Benes P, Smarda J c-Myb regulates NOX1/p38 to control survival of colorectal carcinoma cells. Cell Signal 2016-08-01 [PMID: 27107996]
Kwon J, Wang A, Burke DJ et al. Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration. Free Radic. Biol. Med. 2016-04-14 [PMID: 27094494] (IP, WB, Human) IP, WB
Human
Oxidase N, Fibrosis IC, Pathways S. NADPH Oxidase 4 Induces Cardiac Fibrosis andHypertrophy Through Activating Akt/mTOR and NFkBSignaling Pathways Circulation. 2014-12-12 [PMID: 25589557] (WB, Mouse) WB
Mouse
Wu CH, Tang SC, Wang PH et al. Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation. J Biol Chem 2012-07-01 [PMID: 22648416] (WB, Human) WB
Human
Dvoriantchikova G, Grant J, Santos AR et al. Neuronal NAD(P)H oxidases contribute to ROS production and mediate RGC death after ischemia. Invest Ophthalmol Vis Sci 2012-05-01 [PMID: 22467573] (WB, Human) WB
Human
Musset B, Clark RA, DeCoursey TE et al. NOX5 in human spermatozoa: expression, function, and regulation. J Biol Chem 2012-03-01 [PMID: 22291013] (WB, Human) WB
Human
Cao JY, Mansouri S, Frappier L. Changes in the Nasopharyngeal Carcinoma Nuclear Proteome Induced by the EBNA1 Protein of Epstein-Barr Virus Reveal Potential Roles for EBNA1 in Metastasis and Oxidative Stress Responses. J Virol. 2011-10-19 [PMID: 22013061]
Balasubramaniyan V, Wright G, Sharma V et al. Ammonia reduction with Ornithine phenylacetate restores brain eNOS activity via the DDAH-ADMA pathway in bile duct ligated cirrhotic rats. American Journal of Physiology, Gastrointestinal and Liver Physiology. 2011-09-08 [PMID: 21903766]
Show All 34 Publications.
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Review for NOX1 Antibody (NBP1-31546) (1)
5 1
Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
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reviewed by:
Flow
Human
01/24/2019
Summary
Application Flow Cytometry Sample Tested Platelets Species Human Lot 42214
Comments
Comments Platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer.
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FAQs for NOX1 Antibody (NBP1-31546) (0)
![Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. pSample: Paraffin-embedded human lung carcinoma.NOX1 antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/NOX1-Antibody-Immunohistochemistry-Paraffin-NBP1-31546-img0005.jpg "Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. pSample: Paraffin-embedded human lung carcinoma.NOX1 antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
