Novus Biologicals products are now on bio-techne.com

Dtk/TYRO3 Antibody - BSA Free

Images

 
Western Blot: Dtk/TYRO3 Antibody [NBP1-28635] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.
Immunocytochemistry/ Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of HELA cells using NBP128635 (Green) at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody. ...read more
Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow (Intracellular): Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on U-937 cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.19 mg/ml

Order Details

Dtk/TYRO3 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]
Localization
Cell membrane; Single-pass type I membrane protein.
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TYRO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry reported in scientific literature (PMID 27034374)
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 2 ug/ml
Application Notes
In Western blot, a band can be seen at ~96 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
Dtk/TYRO3 Peptide (NBP1-28635PEP)
Publications
Read Publications using
NBP1-28635 in the following applications:

  • 3 publications

Reactivity Notes

Immunogen sequence has 94% identity to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.19 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dtk/TYRO3 Antibody - BSA Free

  • Brt
  • BYK
  • Dtk
  • EC 2.7.10
  • EC 2.7.10.1
  • FLJ16467
  • Rse
  • Sky
  • Tif
  • TYRO3 protein tyrosine kinase
  • Tyro3
  • Tyrosine-protein kinase byk
  • Tyrosine-protein kinase DTK
  • tyrosine-protein kinase receptor TYRO3
  • Tyrosine-protein kinase RSE
  • Tyrosine-protein kinase SKY

Background

TYRO3 (tyrosine-protein kinase receptor TYRO3) is a RTK which facilitate transduction of signals from extracellular matrix (ECM) into cytoplasm via its direct interaction with multiple ligands such as TUB, TULP1 or GAS6 for the regulation of multiple biological processes such as cell survival, migration and differentiation. TYRO3-ligand binding stimulates its dimerization as well as autophosphorylation on its intracellular domain that provides docking sites for the molecules of downstream signaling. Once stimulated, TYRO3 interacts with PIK3R1 leading to increased PI3K activity followed by AKT signaling activation including NFkB nuclear translocation mediated up-regulation of transcription of NFkB's target genes. TYRO3 signaling events are critical to neuron protection from excitotoxic injury, platelet aggregation, cytoskeletal reorganization, inhibition of TLRs-mediated innate immune response via STAT1 activation followed by production of cytokine signaling suppressors (SOCS1 and SOCS3). TYRO3 is highly expressed during CNS neurogenesis with distinct patterns in adult brain and has been closely related to CNS immunodysfunction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF854
Species: Mu
Applications: IHC, WB
885-GSB
Species: Hu
Applications: BA
AF591
Species: Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC
AF5534
Species: Hu
Applications: Flow, IHC, WB
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
664-LI
Species: Hu
Applications: BA
NBP1-91258
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
H00054778-M05
Species: Hu
Applications: ELISA, ICC/IF, KD, WB
AF2377
Species: Mu
Applications: IHC, Simple Western, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF231
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
NBP1-82458
Species: Hu
Applications: IHC, IHC-P
NBP1-57508
Species: Hu
Applications: IHC, IHC-P, WB
M6000B
Species: Mu
Applications: ELISA

Publications for Dtk/TYRO3 Antibody (NBP1-28635)(4)

Reviews for Dtk/TYRO3 Antibody (NBP1-28635) (0)

There are no reviews for Dtk/TYRO3 Antibody (NBP1-28635). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for Dtk/TYRO3 Antibody (NBP1-28635): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dtk/TYRO3 Antibody (NBP1-28635) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Dtk/TYRO3 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol TYRO3
Entrez
Uniprot