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GPER/GPR30 Antibody [Unconjugated]

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Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GPER at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) followed by HRP-conjugated ...read more
HEK293 human embryonic kidney cell line transfected with (A) human GPER/GRP30 or (B) irrelevant transfectants, and eGFP was stained with Goat Anti-Human GPER/GPR30 Affinity-Purified Polyclonal Antibody (Catalog # ...read more
GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) at 10 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF5534-SP
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GPER/GPR30 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GPER
Met1-Ser62
Accession # Q99527
Specificity
Detects human GPER in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GPR144, GPR115, and GPR124 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPER
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5534 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPER/GPR30 Antibody [Unconjugated]

  • CEPR
  • CEPRG-protein coupled receptor 30
  • Chemoattractant receptor-like 2
  • chemokine receptor-like 2
  • CMKRL2
  • CMKRL2MGC99678
  • constitutively expressed peptide-like receptor
  • DRY12
  • DRY12IL8-related receptor DRY12
  • FEG-1
  • FEG-1mER
  • Flow-induced endothelial G-protein coupled receptor 1
  • G protein-coupled estrogen receptor 1
  • G protein-coupled receptor 30
  • GPCR-BR
  • GPER
  • GPR30
  • GPR30GPCR-Br
  • G-protein coupled estrogen receptor 1
  • heptahelix receptor
  • LERGU
  • LERGU2
  • leucine rich protein in GPR30 3'UTR
  • LyGPR
  • Lymphocyte-derived G-protein coupled receptor
  • Membrane estrogen receptor

Background

GPER (G-Protein Coupled Estrogen Receptor 1; also GPR30, DRY12 and mER) is a 44 kDa, seven transmembrane (TM) member of the GPR-1 family of molecules. It is ubiquitously expressed, appearing on/in neurons, monocytes and endothelial cells. Its exact location is unclear; it has been described in both the cell membrane and ER, but not by all investigators. Human GPER is 375 amino acids (aa) in length. It contains an N-terminal extracellular region (aa 1‑62), a series of seven TM domains (aa 63‑327), and a C-terminal cytoplasmic tail (aa 328‑375). The initial function attributed to GPER was that of a membrane receptor for estrogen. This is in dispute. There are two potential splice variants for GPER. One shows a deletion of aa 32-49, while a second shows a 99 aa substitution for aa 308‑375. Over aa 1‑62, human GPER shares 57% aa identity with mouse GPER.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GPER/GPR30
Antibody [Unconjugate
AF5534
GPER/GPR30 Antibody [Unconjugated]
Species: Hu
Applications: WB, Flow, IHC

Publications for GPER/GPR30 Antibody (AF5534)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


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IHC
(1)
IHC-Fr
(1)
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(1)
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Human
(3)
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Showing Publications 1 - 9 of 9.
Publications using AF5534 Applications Species
Duo Zhang, Jinpeng Wang, Hong Chen, Shunchao Yan Cytoplasmic G Protein-Coupled Estrogen Receptor 1 as a Prognostic Indicator of Breast Cancer: A Meta-Analysis Technol Cancer Res Treat 10/18/2022 [PMID: 36254557]
M Bubb, AL Beyer, P Dasgupta, D Kaemmerer, J Sänger, K Evert, RM Wirtz, S Schulz, A Lupp Assessment of G Protein-Coupled Oestrogen Receptor Expression in Normal and Neoplastic Human Tissues Using a Novel Rabbit Monoclonal Antibody International Journal of Molecular Sciences, 2022-05-06;23(9):. 2022-05-06 [PMID: 35563581] (IHC, Human) IHC Human
Soma Godó, Klaudia Barabás, Ferenc Lengyel, Dávid Ernszt, Tamás Kovács, Miklós Kecskés et al. Single-Molecule Imaging Reveals Rapid Estradiol Action on the Surface Movement of AMPA Receptors in Live Neurons Frontiers in Cell and Developmental Biology 9/23/2021 [PMID: 34631701]
J Tutzauer, M Sjöström, PO Bendahl, L Rydén, M Fernö, LMF Leeb-Lundb, S Alkner Plasma membrane expression of G protein-coupled estrogen receptor (GPER)/G protein-coupled receptor 30 (GPR30) is associated with worse outcome in metachronous contralateral breast cancer PLoS ONE, 2020-04-17;15(4):e0231786. 2020-04-17 [PMID: 32302351] (Human) Human
Rodrigo Azevedo Loiola, Edward S. Wickstead, Egle Solito, Simon McArthur Estrogen Promotes Pro-resolving Microglial Behavior and Phagocytic Cell Clearance Through the Actions of Annexin A1 Front Endocrinol (Lausanne) 6/26/2019 [PMID: 31297095]
Viviana Bustos, Áine M. Nolan, Anke Nijhuis, Harry Harvey, Alexandra Parker, Richard Poulsom et al. GPER mediates differential effects of estrogen on colon cancer cell proliferation and migration under normoxic and hypoxic conditions Oncotarget 10/13/2017 [PMID: 29137421]
Broselid S, Berg K, Chavera T, Kahn R, Clarke W, Olde B, Leeb-Lundberg L G protein-coupled receptor 30 (GPR30) forms a plasma membrane complex with membrane-associated guanylate kinases (MAGUKs) and protein kinase A-anchoring protein 5 (AKAP5) that constitutively inhibits cAMP production. J Biol Chem, 2014-06-24;289(32):22117-27. 2014-06-24 [PMID: 24962572] (IHC-Fr, Western Blot, Human) IHC-Fr, Western Blot Human
Kolkova Z, Casslen V, Henic E, Ahmadi S, Ehinger A, Jirstrom K, Casslen B The G protein-coupled estrogen receptor 1 (GPER/GPR30) does not predict survival in patients with ovarian cancer. J Ovarian Res, 2012-03-18;5(0):9. 3/18/2012 [PMID: 22424333]
Z. Kolkova, V. Noskova, A. Ehinger, S. Hansson, B. Casslen G protein-coupled estrogen receptor 1 (GPER, GPR 30) in normal human endometrium and early pregnancy decidua Molecular Human Reproduction 10/1/2010 [PMID: 20508064]

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Bioinformatics

Gene Symbol GPER
Uniprot