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DRP1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining in human cerebral cortex and pancreas tissues.. Corresponding DNM1L RNA-seq data are presented for the same tissues.
Western Blot: DRP1 Antibody [NBP2-34205] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody [NBP2-34205] - Staining of human cell line U-251 MG shows localization to cytosol and vesicles.
Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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DRP1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: EADGKVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCE
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNM1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Rat (85%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DRP1 Antibody

  • DLP1
  • DNM1L
  • DRP1
  • DRP1Dnm1p/Vps1p-like protein
  • DVLP
  • DVLPEC 3.6.5.5
  • Dymple
  • DYMPLEDYNIV-11
  • dynamin 1-like
  • Dynamin family member proline-rich carboxyl-terminal domain less
  • dynamin-1-like protein
  • Dynamin-like protein 4
  • Dynamin-like protein IV
  • Dynamin-like protein
  • Dynamin-related protein 1
  • FLJ41912
  • HdynIV
  • VPS1

Background

DRP1 is a member of the dynamin GTPase superfamily, which that contributes to mitochondrial division in mammalian cells. DRP1 plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses. Therefore, DRP1 provides a genetic tool to assess the role of mitochondria at synapses.

The DRP1 gene has 3 alternatively spliced transcripts encoding different isoforms, which are alternatively polyadenylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DRP1 Antibody (NBP2-34205) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DRP1 Antibody (NBP2-34205) (0)

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Secondary Antibodies

 

Isotype Controls

Additional DRP1 Products

Research Areas for DRP1 Antibody (NBP2-34205)

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Blogs on DRP1.

Methamphetamine with HIV induces mitochondrial dysfunction and neuronal injury through oxidative stress
By Jamshed Arslan, Pharm. D., PhD. December 1 is the World AIDS Day. Despite the combination antiretroviral therapy, 10-25% of Human Immunodeficiency Virus (HIV)-positive individuals report neurocognitive impairm...  Read full blog post.

Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles
By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox...  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Bioinformatics

Gene Symbol DNM1L
Uniprot