![Immunocytochemistry/Immunofluorescence: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:4500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25563_rabbit-mff-mab-hl1311-azide-and-bsa-free-2120249383144.jpg "Immunocytochemistry/Immunofluorescence: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:4500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1311 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MFF. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MFF |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MFF Antibody (NBP3-25563)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MFF |
![Immunohistochemistry-Paraffin: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25563_rabbit-mff-mab-hl1311-azide-and-bsa-free-2120249374998.jpg "Immunohistochemistry-Paraffin: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - U87-MG whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1311] (NBP3-25563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25563_rabbit-mff-mab-hl1311-azide-and-bsa-free-212024941464.jpg "Western Blot: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - U87-MG whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1311] (NBP3-25563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25563_rabbit-mff-mab-hl1311-azide-and-bsa-free-2120249351344.jpg "Immunohistochemistry-Paraffin: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Immunohistochemistry-Paraffin MLKL [p Thr357] Antibody (954724) - Azide and BSA Free](https://images.novusbio.com/images/MLKL-p-Thr357-Antibody-954724-Immunohistochemistry-Paraffin-MAB91871-img0002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")