SMAC/Diablo Antibody

Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using NB100-56311	Applications	Species
Qiu W, Liu H, Sebastini A et al. An apoptosis-independent role of SMAC in tumor suppression. Oncogene. 2013-05-09 [PMID: 22751125] (IHC-P, WB, Mouse) Details: IMGENEX Product Cited: SMAC (IMG-248A). 1. IHC(paraffin): Human colon tumors, Fig 1A 2. WB: Mouse colonic mucosa from WT and SMACK knockout mice, Figs 2D. 3. WB: Mouse small intestinal mucosa from WT and SMACK knockout mice, Fig 6A 4. WB: HCT116 cell line	IHC-P, WB	Mouse
Endo Kazuya, Kohnoe Shunji, Watanabe Akihiro et al. Clinical significance of Smac/DIABLO expression in colorectal cancer. Oncol Rep. 2009-02-01 [PMID: 19148507] (Human)		Human
Conus S, Perozzo R, Reinheckel T et al. Caspase-8 is activated by cathepsin D initiating neutrophil apoptosis during the resolution of inflammation. J Exp Med. 2008-03-17 [PMID: 18299403] Details: IF (confocal microscopy on human neutrophils), Fig. 2B.
Ren Y, Akyurek N, Schlette E et al. Expression of Smac/DIABLO in B-cell non-Hodgkin and Hodgkin lymphomas. Hum Pathol. 2006-11-01 [PMID: 16949641] (IHC-P, WB)	IHC-P, WB
Liu T, Biddle D, Hanks AN et al. Activation of dual apoptotic pathways in human melanocytes and protection by survivin. J Invest Dermatol. 2006-10-01 [PMID: 16728972] (WB, Human) Details: Smac/DIABLO (IMG-248A). WB: Mitochondrial and cytosolic fractions of human melanocytes treated with either UV radiation (UVB), 4-tert-butylphenol (4-TBP), or cisplatin (CP), Fig 4A.	WB	Human
Qin JZ, Ziffra J, Stennett L et al. Proteasome inhibitors trigger NOXA-mediated apoptosis in melanoma and myeloma cells. Cancer Res. 2005-07-15 [PMID: 16024630]
Berthier A, Lemaire-Ewing S, Prunet C et al. 7-Ketocholesterol-induced apoptosis. Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways. FEBS J. 2005-06-01 [PMID: 15955068] (WB) Details: Smac/DIABLO (IMG-248A). WB: THP-1 cells treated with 7-ketocholestrol, Fig 4.	WB
Lee JC, Lee CH, Su CL et al. Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells. Carcinogenesis. 2005-10-01 [PMID: 15905197] (WB) Details: Caspase-3 (IMG-144A) [WB, Fig 3A (HT-29 and HCT 116 cells)].	WB
Mizutani Y, Nakanishi H, Yamamoto K et al. Downregulation of Smac/DIABLO expression in renal cell carcinoma and its prognostic significance. J Clin Oncol. 2005-01-20 [PMID: 15572731] (WB, Human) Details: Smac/DIABLO (IMG-248). WB: Human renal cell carcinoma (RCC) normal kidney, Fig 1 & 2.	WB	Human
Hu P, Han Z, Couvillon AD, Exton JH. Critical role of endogenous Akt/IAPs and MEK1/ERK pathways in counteracting endoplasmic reticulum stress-induced cell death. J Biol Chem. 2004-11-19 [PMID: 15339911] (ICC/IF) Details: IF/ICC: MCF-7 cells treated with thapsigargin, Fig 7A.	ICC/IF
Qin JZ, Stennett L, Bacon P et al. p53-independent NOXA induction overcomes apoptotic resistance of malignant melanomas. Mol Cancer Ther. 2004-08-01 [PMID: 15299072] (WB) Details: Smac/DIABLO (IMG-248A). WB: Human melanoma (RJ002L) cells treated with y-secretase tripeptide inhibitor (GSI), Fig 3. Note: GSI triggers release of SMAC from mitochondria into cytoplasm in RJ002L cells after 6 hours, Fig 3.	WB
Wu J, Parungo C, Wu G et al. PR39 inhibits apoptosis in hypoxic endothelial cells: role of inhibitor apoptosis protein-2. Circulation. 2004-04-06 [PMID: 15023888] (WB) Details: Smac/DIABLO (IMG-248). WB: Bovine aortic endothelial cells (BAEC) transfected with pcDNA4-Smac, Fig 6. Note: The specificity of the Smac/DIABLO was validated by WB in Smac transfected BAEC's, Fig 6.	WB
Liu T, Brouha B, Grossman D. Rapid induction of mitochondrial events and caspase-independent apoptosis in Survivin-targeted melanoma cells. Oncogene. 2004-01-08 [PMID: 14712209]
Liu J, Dai Q, Chen J et al. Phospholipid scramblase 3 controls mitochondrial structure, function, and apoptotic response. Mol Cancer Res. 2003-10-01 [PMID: 14573790]
Yoo NJ, Kim HS, Kim SY et al. Immunohistochemical analysis of Smac/DIABLO expression in human carcinomas and sarcomas. APMIS. 2003-03-01 [PMID: 12752217]
Vucic D, Deshayes K, Ackerly H et al. SMAC negatively regulates the anti-apoptotic activity of melanoma inhibitor of apoptosis (ML-IAP). J Biol Chem. 2002-04-05 [PMID: 11801603]
Show All 16 Publications. Collapse Publications.

Array NB100-56311

• SMAC/Diablo Antibodies
• SMAC/Diablo ELISA Kits
• SMAC/Diablo Lysate
• SMAC/Diablo Proteins
• SMAC/Diablo Proteins and Enzyme