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Doublecortin Antibody (BLR168J)

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Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. used at 1:1000. Secondary: HRP-conjugated goat ...read more
Immunocytochemistry: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate DAB
Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB
Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB
Immunoprecipitation- Doublecortin Antibody (BLR168J)-NBP3-14742-Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit antiDoublecortin ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
BLR168J
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Doublecortin Antibody (BLR168J) Summary

Additional Information
Recombinant Monoclonal
Immunogen
between 200 and 250
Clonality
Monoclonal
Host
Rabbit
Gene
DCX
Purity
>95%
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 to 1:500
  • Immunohistochemistry-Paraffin 1:100 to 1:500
  • Immunoprecipitation 20 µl/1 mg lysate
  • Western Blot 1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Purity
>95%

Alternate Names for Doublecortin Antibody (BLR168J)

  • DBCN
  • Doublecortex
  • Doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • LISX
  • Lis-X
  • X-linked (doublecortin)

Background

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DCX