MGMT Antibody (MT 23.2) - BSA Free

MGMT (6-O-methylguanine-DNA methyltransferase) is a DNA repair enzyme with the ability to protect cells from carcinogenic effects of alkylating agents by removing adducts from the O(6) position of guanine. MGMT repairs alkylated guanine residues in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme, and in this so called 'suicidal reaction', the enzyme is irreversibly inactivated. Localized in the nucleus, MGMT is ubiquitously expressed in normal human tissues with overexpression observed in several types of cancerous cells wherein it provides resistance against alkylating drugs. However, it is found epigenetically silenced in some cancers that include lymphoma, breast and prostate cancer, as well as retinoblastoma, wherein this silencing is thought to be due to promoter methylation which interferes with transcriptional factors binding. MGMT silencing has been linked to mutations in other tumor-related genes including silencing of p53 (non-small cell lung cancer and astrocytic tumors), k-ras gene mutations (gastric and colorectal cancer), and methylation of CDKN1A gene (encodes p21) as well as CDKN2A gene (encodes p16).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NB100-168	Applications	Species
Powell G, Pavlovic Djuranovic S, Djuranovic S. Gene dosage effects of poly(A) track-engineered hypomorphs Molecular therapy. Nucleic acids 2021-12-03 [PMID: 34729253] (WB)	WB
Fang Q, Inanc B, Schamus S et al. HSP90 regulates DnA repair via the interaction between XRCC1 and DnA polymerase B. Nat Commun. 2014-11-26 [PMID: 25423885] (WB)	WB
Sav A, Atinoz M, Rotondo F et al. Tumor-to-tumor metastasis: lung adenocarcinoma into a clinically non-functioning gonadotroph pituitary adenoma: a rare case. J Neurol Sci Turk 2017-04-21 (IF/IHC, Human)	IF/IHC	Human
Sari R, Altinoz Ma, Ozlu Ebk Et Al. Treatment Strategies for Dopamine Agonist-Resistant and Aggressive Prolactinomas: A Comprehensive Analysis of the Literature Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 2021-07-01 [PMID: 34282593]
KotSarenko K, Lylo V, Ruban T et al. Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro : Effect of Some Growth Factors and Cytokines. Biochem. Genet. 2018-03-27 [PMID: 29589213] (Human)		Human
Altinoz MA, Elmaci I, Bolukbasi FH et al. MGMT gene variants, temozolomide myelotoxicity and glioma risk. A concise literature survey including an illustrative case. J Chemother. 2017-04-23 [PMID: 28436299] (Human)		Human
Oktay Y, ulgen E, Can O et al. IDH-mutant glioma specific association of rs55705857 located at 8q24.21 involves MYC deregulation. Sci Rep 2016-06-10 [PMID: 27282637] (IF/IHC)	IF/IHC
Stepanenko AA, Andreieva SV, Korets KV et al. Temozolomide promotes genomic and phenotypic changes in glioblastoma cells. Cancer Cell Int 2016-05-05 [PMID: 27158244] (WB)	WB
Kewitz S, Stiefel M, Kramm CM, Staege MS. Impact of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and MGMT expression on dacarbazine resistance of Hodgkin's lymphoma cells. Leuk Res. 2013-11-13 [PMID: 24284332] (WB, Human)	WB	Human
Persano L, Pistollato F, Rampazzo E et al. BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1 alpha stability and MGMT expression Cell Death Dis 2012-10-18 [PMID: 23076220] (WB, Human)	WB	Human
Doll C, Craighead P, Lees-Miller S, Demetrick D, Eliasziw M, Magliocco A. Expression of Mgmt in Cervical Cancer: a Potential Biomarker for Response to Radiation in Cervical Cancer. 21st Annual Scientific Meeting of the Canadian Association of Radiation Oncologists joint with Canadian Organization of Medical; Toronto; Canada. 2007-10-09
Lun X, Wells J C et al. Disulfiram when Combined with Copper Enhances the Therapeutic Effects of Temozolomide for the Treatment of Glioblastoma. Clin Cancer Res 2016-01-08 [PMID: 27006494] (WB, Human)	WB	Human
Lylo, V V, Matsevich L L, Kotsarenko, E V et al. Activation of Gene Expression of the O6 Methylguanine DNA Transferase Repair Enzyme upon the Influence of EMAP II Cytokine in Human Cells In Vitro. Cytology and Genetics 2011-01-01 [PMID: 22329163]
Herzig MC, Hildreth K, Huamani J, Perez M, Goins BA, McMahan CA, Reddick RL, Walter CA. Human O(6) -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Mol Carcinog. 2011-12-27 [PMID: 22213062] (WB, Mouse)	WB	Mouse
Della Puppa A, Persano L, Masi G et al. MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients? . J Neurooncol. 2011-07-02 [PMID: 21725802]
Tang JB, Svilar D, Trivedi RN, Wang XH, Goellner EM, Moore B, Hamilton RL, Banze LA, Brown AR, Sobol RW. N-methylpurine DNA glycosylase and DNA polymerase beta modulate BER inhibitor potentiation of glioma cells to temozolomide. Neuro Oncol;13(5):471-86. 2011-05-01 [PMID: 21377995] (WB, Human)	WB	Human
Pistollato F, Abbadi S, Rampazzo E et al. Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells 2010-05-01 [PMID: 20309962] (WB, IHC-P, Human)	WB, IHC-P	Human
Cai S, Xu Y, Cooper RJ et al. Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents. Cancer Res;65(8):3319-27. 2005-04-15 [PMID: 15833865] (WB)	WB
Zuo C et al. O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev;13(6):967-75. 2004-06-01 [PMID: 15184253] (IHC-P, Human)	IHC-P	Human
Roth TJ, Xu Y, Luo M et al. Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection. Cancer Gene Ther;10(8):603-10. 2003-08-01 [PMID: 12872142] (WB)	WB
Liu L, Spiro TP, Qin X, Majka S, Haaga J, Schupp J, Willson JK, Gerson SL. Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanine. Clin Cancer Res;7(8):2318-24. 2001-08-01 [PMID: 11489807] (IF/IHC, Human)	IF/IHC	Human
Show All 21 Publications. Collapse Publications.

Array NB100-168

• MGMT Antibodies
• MGMT ELISA Kit
• MGMT Lysate
• MGMT Proteins