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![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0005.jpg "Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16182-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - Human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP2-16182-img0002.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0001.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -Rat colon.DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-immunohistochemistry-paraffin-992024123530..jpg)
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-immunocytochemistry-immunofluorescence-992024123725..jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-910202420134047.jpg)
![Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-91020242041277.jpg)
![Western Blot: DNA Ligase IV Antibody [NB110-57379] - HepG2 cell lysate.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NB110-57379-img0005.jpg "Western Blot: DNA Ligase IV Antibody [NB110-57379] - HepG2 cell lysate.")
![Immunohistochemistry: DNA Ligase IV Antibody [NB110-57379] - Immunohistochemical staining of human pancreatic tissue using NB110-57379 at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-NB110-57379-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0005.jpg "Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells and smooth muscle.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0006.jpg)
![Western Blot: DNA Ligase IV Antibody [NBP3-35747] - Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35747_rabbit-dna-ligase-iv-pab-91220241418132.jpg "Western Blot: DNA Ligase IV Antibody [NBP3-35747] - Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: DNA Ligase IV Antibody [NBP3-35747] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DNA Ligase IV Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35747_rabbit-dna-ligase-iv-pab-9122024141817.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-68729] - Staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68729-img0001.jpg "Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-68729] - Staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.")
![Western Blot: DNA Ligase IV Overexpression Lysate (Adult Normal) [NBL1-12521] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase IV.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12521-img0002.jpg "Western Blot: DNA Ligase IV Overexpression Lysate (Adult Normal) [NBL1-12521] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase IV.")
![Western Blot: DNA Ligase IV Overexpression Lysate (Adult Normal) [NBL1-12522] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase IV.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12522-img0002.jpg "Western Blot: DNA Ligase IV Overexpression Lysate (Adult Normal) [NBL1-12522] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase IV.")
![Western Blot: DNA Ligase IV Overexpression Lysate (Adult Normal) [NBP2-11429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase IV.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11429-img0001.jpg "Western Blot: DNA Ligase IV Overexpression Lysate (Adult Normal) [NBP2-11429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase IV.")
![Western Blot: DNA Ligase IV Antibody (2T6J2) [NBP3-15379] - Western blot analysis of extracts of various cell lines, using DNA Ligase IV Rabbit mAb (NBP3-15379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-2T6J2-Western-Blot-NBP3-15379-img0001.jpg "Western Blot: DNA Ligase IV Antibody (2T6J2) [NBP3-15379] - Western blot analysis of extracts of various cell lines, using DNA Ligase IV Rabbit mAb (NBP3-15379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: DNA Ligase IV Antibody (S08-3B4) [NBP3-14926] - Western blot detection of DNA Ligase IV in K562, U251MG, HepG2, MCF7 cell lysates using DNA Ligase IV Rabbit mAb (1:500 diluted). Predicted band size: 104kDa. Observed band size: 104kDa.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-S08-3B4-Western-Blot-NBP3-14926-img0001.jpg "Western Blot: DNA Ligase IV Antibody (S08-3B4) [NBP3-14926] - Western blot detection of DNA Ligase IV in K562, U251MG, HepG2, MCF7 cell lysates using DNA Ligase IV Rabbit mAb (1:500 diluted). Predicted band size: 104kDa. Observed band size: 104kDa.")
![Sandwich ELISA: DNA Ligase IV Antibody (2D2) [H00003981-M02] - Detection limit for recombinant GST tagged LIG4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-2D2-Sandwich-ELISA-H00003981-M02-img0002.jpg "Sandwich ELISA: DNA Ligase IV Antibody (2D2) [H00003981-M02] - Detection limit for recombinant GST tagged LIG4 is approximately 1ng/ml as a capture antibody.")