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DNA Ligase IV Antibody

Images

 
Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells and smooth muscle.
Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DNA Ligase IV Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIG4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DNA Ligase IV Protein (NBP1-87405PEP)
Publications
Read Publication using NBP1-87405.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23405159). Mouse (83%), Rat (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNA Ligase IV Antibody

  • DNA joinase
  • DNA ligase 4
  • DNA Ligase IV
  • DNA repair enzyme
  • EC 6.5.1.1
  • LIG4
  • ligase IV, DNA, ATP-dependent
  • Polydeoxyribonucleotide synthase [ATP] 4
  • polynucleotide ligase
  • sealase

Background

LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Ligase IV Antibody (NBP1-87405)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LIG4