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DNA Ligase IV Antibody (S08-3B4)

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Western Blot: DNA Ligase IV Antibody (S08-3B4) [NBP3-14926] - Western blot detection of DNA Ligase IV in K562, U251MG, HepG2, MCF7 cell lysates using DNA Ligase IV Rabbit mAb (1:500 diluted). Predicted band size: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
S08-3B4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DNA Ligase IV Antibody (S08-3B4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein of human DNA Ligase IV (Uniprot#: P49917)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LIG4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1/1000
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for DNA Ligase IV Antibody (S08-3B4)

  • DNA joinase
  • DNA ligase 4
  • DNA Ligase IV
  • DNA repair enzyme
  • EC 6.5.1.1
  • LIG4
  • ligase IV, DNA, ATP-dependent
  • Polydeoxyribonucleotide synthase [ATP] 4
  • polynucleotide ligase
  • sealase

Background

LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIG4