![Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of various cell lines, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NBP2-92984-img0001.jpg "Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of various cell lines, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 201-300 of human DGAT1 (NP_036211.2). YELNFPRSPRIRKRFL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DGAT1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DGAT1 Antibody (NBP2-92984)Find related products by research area.
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Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat... Read full blog post. |
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| Gene Symbol | DGAT1 |
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Analysis of PC-12 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92984-img0005.jpg "Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Analysis of PC-12 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.")
![Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of Mouse liver, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DGAT1 Antibody-Western Blot-NBP2-92984-img0002.jpg "Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of Mouse liver, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Analysis of MCF7 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92984-img0003.jpg "Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Analysis of MCF7 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Aalysis of NIH/3T3 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92984-img0004.jpg "Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Aalysis of NIH/3T3 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")