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![Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NB110-41487-img0002.jpg "Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.")
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB110-41487] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-DGAT1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41487-img0003.jpg)
![Western Blot: DGAT1 Antibody [NB110-41487] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Pictured is protein abundance of DGAT1. In the figure panel, the data is expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blot shown inset above the figure panel. DGAT = diacylglycerol acyltransferase; AU = arbitrary units. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NB110-41487-img0004.jpg)
![Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NB100-57086-img0006.jpg "Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB100-57086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57086-img0008.jpg)
![Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086] - Staining of paraffin embedded Human Colon. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunohistochemistry-Paraffin-NB100-57086-img0005.jpg)
![Flow Cytometry: DGAT1 Antibody [NB100-57086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Flow-Cytometry-NB100-57086-img0007.jpg)
![Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of various cell lines, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NBP2-92984-img0001.jpg "Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of various cell lines, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Analysis of PC-12 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92984-img0005.jpg)
![Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of Mouse liver, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DGAT1 Antibody-Western Blot-NBP2-92984-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Analysis of MCF7 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92984-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Aalysis of NIH/3T3 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DGAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92984-img0004.jpg)
![Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22666_rabbit-dgat1-mab-sr1829-57202316494249.jpg "Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.")
![Western Blot: DGAT1 Antibody (JE34-88) [NBP3-32265] - Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: MCF7 cell lysate (RIPA lysis) Lane 3: MCF7 cell lysate (hot lysis) Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32265) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32265_rabbit-dgat1-mab-je34-88-278202416331280.jpg "Western Blot: DGAT1 Antibody (JE34-88) [NBP3-32265] - Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: MCF7 cell lysate (RIPA lysis) Lane 3: MCF7 cell lysate (hot lysis) Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32265) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DGAT1 Antibody (JE34-88) [NBP3-32265] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32265) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32265_rabbit-dgat1-mab-je34-88-29820241492691.jpg)
![Western Blot: DGAT1 Antibody [NBP3-10973] - Western blot analysis of DGAT1 in Mouse Heart lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/DGAT1-Antibody-Western-Blot-NBP3-10973-img0001.jpg "Western Blot: DGAT1 Antibody [NBP3-10973] - Western blot analysis of DGAT1 in Mouse Heart lysates. Antibody dilution at 1.0ug/ml")
![ELISA: Human DGAT1 ELISA Kit (Colorimetric) [NBP3-31950] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31950_human-dgat1-elisa-kit-colorimetric-206202415301560.png "ELISA: Human DGAT1 ELISA Kit (Colorimetric) [NBP3-31950] - Standard Curve Reference")
![ELISA: Human DGAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31951] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31951_human-dgat1-ready-to-use-elisa-kit-colorimetric-206202415273740.png "ELISA: Human DGAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31951] - Standard Curve Reference")