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DDX3 Antibody - Azide and BSA Free

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Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of U-2 OS cells using DDX3Y . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat testis using DDX3Y antibody at dilution of 1:100 (40x lens).
Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of C6 cells using DDX3Y . Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of L929 cells using DDX3Y . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat brain using DDX3Y antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse heart using DDX3Y antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse brain using DDX3Y antibody at dilution of 1:100 (40x lens).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-92850-0.02ml
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DDX3 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1). MSHVVVKNDPELDQQLANLDLNSEKQSGGASTASKGRYIPPHLRNREASKGFHDKDSSGWSCSKDKDAYSSFGSRDSRGKPGYFSERGSGSRGRFDDRGRSDYDGIGNRERPGFGRFERSGHSRWCDKSVEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGSNCPPHIENFSDIDMGEIIMGNIELTRYTRPTPVQKHAIP
Specificity
This antibody was raised to human DDX3Y fragment, however it is known to crossreact with DDX3X.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX3
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for DDX3 Antibody - Azide and BSA Free

  • DBXCAP-Rf
  • DDX14
  • DDX3DEAD/H box-3
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
  • DEAD box protein 3, X-chromosomal
  • DEAD box, X isoform
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
  • EC 3.6.1
  • EC 3.6.4.13
  • helicase like protein 2
  • Helicase-like protein 2
  • HLP2ATP-dependent RNA helicase DDX3X

Background

DDX3X, also known as ATP-dependent RNA helicase DDX3X, consists of a 73.2 kDa and a 71.4 kDa isoform which is involved in multiple gene expression steps, including transcription and translation. Current research is linking the protein to diseases and disorders such as hypoxia, hepatitis, malaria, infertility, carcinoma, breast cancer, and prostate cancer. The protein interacts in translational control, cell cycle checkpoint control, and receptor signaling pathways with other proteins such as ACTB, TBK1, NFKB2, NXF1, and EIF3B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol DDX3