![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0007.jpg "Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clone | 3E5E2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DDX1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Immunoprecipitation reported in scientific literature (PMID:32755435) |
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| Theoretical MW | 82.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Western Blot DDX17 Antibody [Unconjugated]](https://images.novusbio.com/images2/DDX17_AF4530_Western_Blot_5487.jpg)
![Immunohistochemistry DDX17 Antibody [Unconjugated]](https://images.novusbio.com/images2/DDX17_AF4530_Immunohistochemistry_19615.jpg)
![Immunocytochemistry DDX17 Antibody [Unconjugated]](https://images.novusbio.com/images2/DDX17_AF4530_Immunocytochemistry__Immunofluorescence_20569.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Verified Customer |
WB | Human | 05/07/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for DDX1 Antibody (NBP2-61745)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DDX1 |
![Immunocytochemistry/Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunocytochemistry-Immunofluorescence-NBP2-61745-img0003.jpg "Immunocytochemistry/Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunohistochemistry-NBP2-61745-img0005.jpg "Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.")
![Flow Cytometry: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Flow-Cytometry-NBP2-61745-img0002.jpg "Flow Cytometry: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).")
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0006.jpg "Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)")
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0008.jpg "Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate.")
![Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of colon cancer tissues using DDX1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunohistochemistry-NBP2-61745-img0004.jpg "Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of colon cancer tissues using DDX1 mouse mAb with DAB staining.")
![ELISA: DDX1 Antibody (3E5E2) [NBP2-61745] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-ELISA-NBP2-61745-img0001.jpg "ELISA: DDX1 Antibody (3E5E2) [NBP2-61745] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
