DDX3 Antibodies
We offer DDX3 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each DDX3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DDX3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the DDX3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DDX3 polyclonal antibodies and browse our DDX3 monoclonal antibody catalog.
Alternate Names for DDX3 Antibodies
anti-DDX3 antibody, anti-DDX3X antibody, anti-DBXCAP-Rf antibody, anti-DDX14 antibody, anti-DDX3DEAD/H box-3 antibody, anti-DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked antibody, anti-DEAD box protein 3, X-chromosomal antibody, anti-DEAD box, X isoform antibody, anti-DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3 antibody, anti-EC 3.6.1 antibody, anti-EC 3.6.4.13 antibody, anti-helicase like protein 2 antibody, anti-Helicase-like protein 2 antibody, anti-HLP2ATP-dependent RNA helicase DDX3X antibody
14 Results for "ddx3" in Primary Antibodies
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0010.jpg "Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.")
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0006.jpg)
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0002.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0007.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0009.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0008.jpg)
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0005.jpg)
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0001.jpg)
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0003.jpg)
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0004.jpg)
![Western Blot: DDX3 Antibody [NBP1-85291] - Analysis: NBP1-85291 (A) shows similar pattern to independent antibody NBP1-85290 (B)](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP1-85291-img0027.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX3 Antibody [NBP1-85291] - Analysis: NBP1-85291 (A) shows similar pattern to independent antibody NBP1-85290 (B)")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP1-85291] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0019.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse hippocampus shows moderate positivity in a subset of neurons in the brain.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0026.jpg)
![Western Blot: DDX3 Antibody [NBP1-85291] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP1-85291-img0018.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse medulla shows strong cytoplasmic immunoreactivity in neurons of the facial nucleus.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0007.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse thalamus shows positivity in neurons of the anterodorsal thalamic nucleus.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0009.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse midbrain shows neuronal positivity in the red nucleus.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0012.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear immunoreactivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0014.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0020.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0021.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human hippocampus shows moderate positivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0022.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human Fallopian tube shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0023.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0024.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse cerebral cortex shows strong positivity in neurons in the parietal association cortex.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0025.jpg)
![Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0008.jpg "Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DDX3 Antibody [NB200-194] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-194 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-195 and NB 200-196 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0005.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Sample: FFPE section of human lung cancer pleural effusion (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescence (A120-601D4) and DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0006.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of mouse DDX3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX3 Antibody [NB200-194] - Detection of human DDX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX3 antibody NB200-194 (lot NB200-194-2) used for IP at 6 ug per reaction. DDX3 was also immunoprecipitated by a previous lot of this antibody (lot NB200-194-1) and rabbit anti-DDX3 antibody NB200-196. For blotting immunoprecipitated DDX3, NB200-194 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunoprecipitation-NB200-194-img0007.jpg)
![Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-92850-img0008.jpg "Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of U-2 OS cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0003.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat testis using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of C6 cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of L929 cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0002.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat brain using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0004.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse heart using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse brain using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0006.jpg)
![Western Blot: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of human DDX3 using A) a partial recombinant protein (amino acids 24-203) and B) 293 lysate probed with DDX3 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DDX3-Antibody-11F11D10-Western-Blot-NBP2-14848-img0003.jpg "Western Blot: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of human DDX3 using A) a partial recombinant protein (amino acids 24-203) and B) 293 lysate probed with DDX3 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of DDX3 in human MCF-7 and HeLa cells using DDX3 antibody at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/DDX3-Antibody-11F11D10-Flow-Cytometry-NBP2-14848-img0002.jpg)
![Western Blot: DDX3 Antibody [NBP2-16133] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-16133-img0002.jpg "Western Blot: DDX3 Antibody [NBP2-16133] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:2000.")
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-16133] - Analysis of paraformaldehyde-fixed Human ESC, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16133-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-16133] - Immunohistochemical analysis of paraffin-embedded Normal colon, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-16133-img0003.jpg)
![Western Blot: DDX3 Antibody [NBP2-16133] - Sample (20 ug of cell lysate) A: HeLaB: HeLa cytoplasm7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-16133-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-16133] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16133-img0004.jpg)
![Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in NIH/3T3.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Western-Blot-H00008653-M01-img0011.jpg "Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in NIH/3T3.")
![Immunohistochemistry-Paraffin: DDX3 Antibody (2D7) [H00008653-M01] - Analysis of monoclonal antibody to DDX3Y on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Immunohistochemistry-Paraffin-H00008653-M01-img0007.jpg)
![Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in Raw 264.7.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Western-Blot-H00008653-M01-img0010.jpg)
![Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in mouse testis.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Western-Blot-H00008653-M01-img0012.jpg)
![Sandwich ELISA: DDX3 Antibody (2D7) [H00008653-M01] - Detection limit for recombinant GST tagged DDX3Y is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Sandwich-ELISA-H00008653-M01-img0008.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - A. 30 ug Jurkat B. 30 ug Raji C. 30 ug NCI-H929 7.5 % SDS-PAGE DDX3 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-NBP1-32780-img0013.jpg "Western Blot: DDX3 Antibody [NBP1-32780] - A. 30 ug Jurkat B. 30 ug Raji C. 30 ug NCI-H929 7.5 % SDS-PAGE DDX3 antibody dilution: 1:10000")
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP1-32780] - Analysis of paraformaldehyde-fixed HeLa, using DDX3Y antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32780-img0012.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-NBP1-32780-img0009.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - 50 ug Rat testis lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-NBP1-32780-img0010.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - Sample (30 ug of whole cell lysate)A: H1299B: Hep G2 7. 5% SDS PAGE, antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-NBP1-32780-img0011.jpg)
![Western Blot: DDX3 Antibody [NB200-196] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-196 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-194 and NB 200-195 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-196-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX3 Antibody [NB200-196] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-196 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-194 and NB 200-195 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).")
![Western Blot: DDX3 Antibody [NBP2-56579] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-56579-img0003.jpg "Western Blot: DDX3 Antibody [NBP2-56579] - Analysis in human cell line RT-4.")
![Immunohistochemistry: DDX3 Antibody [NBP2-56579] - Immunohistochemical staining of human salivary gland shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NBP2-56579-img0002.jpg)
![Western Blot: DDX3 Antibody [NBP2-92748] - Analysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-92748-img0001.jpg "Western Blot: DDX3 Antibody [NBP2-92748] - Analysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Western Blot: DDX3 Antibody [H00008653-D01P] - Analysis of DDX3Y expression in transfected 293T cell line by DDX3Y polyclonal antibody.Lane 1: DDX3Y transfected lysate(73.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-H00008653-D01P-img0002.jpg "Western Blot: DDX3 Antibody [H00008653-D01P] - Analysis of DDX3Y expression in transfected 293T cell line by DDX3Y polyclonal antibody.Lane 1: DDX3Y transfected lysate(73.20 KDa).Lane 2: Non-transfected lysate.")