DDX1 Antibody Summary
Immunogen |
The immunogen recognized by this antibody maps to a region between residue 650 and the C-terminus (residue 697) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653). |
Predicted Species |
Rat (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
DDX1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 1:100-1:500
- Immunohistochemistry-Paraffin 1:100-1:500
- Immunoprecipitation 3-5 ug/mg lysate
- Western Blot 1:1000-1:10000
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Application Notes |
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Control |
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Publications |
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Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
TBS and 0.1% BSA |
Preservative |
0.09% Sodium Azide |
Concentration |
0.2 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for DDX1 Antibody
Background
DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The family's name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DDX1, was identified in retinoblastoma cell lines, where it is coamplified with MYCN, a member of the MYC transcription factor family. DDX1 is expressed at elevated levels in fetal tissues of neural origin (retina and brain). It is both cytoplasmic and nuclear in DDX1-amplified neuroblastoma and retinoblastoma cell lines. However, it localizes specifically to the nucleus in non-amplified cell lines. Thus, DDX1 is thought to be involved in nuclear RNA metabolism. In addition, the decrease in mean disease-free survival of neuroblastoma patients exhibiting coamplification of DDX1 and MYCN as compared to those exhibiting MYCN amplification indicates a possible role for DDX1 in cancer formation in neural tissues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for DDX1 Antibody (NB200-349)(1)
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Product General Protocols
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Video Protocols
FAQs for DDX1 Antibody (NB200-349) (0)
Control Lysate(s)
Secondary Antibodies
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Isotype Controls
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