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SFPQ Antibody

Images

 
Western Blot: SFPQ Antibody [NBP2-20329] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody diluted by 1:10000.
Immunocytochemistry/ Immunofluorescence: SFPQ Antibody [NBP2-20329] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal diluted at 1:500. Red: ...read more
Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal diluted at 1:500.
Western Blot: SFPQ Antibody [NBP2-20329] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody diluted by 1:10000.
Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Immunohistochemical analysis of paraffin-embedded mouse uterus, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Paraffin-embedded mouse cervix. SFPQ antibody [N2C1], Internal diluted at 1:500.
Immunoprecipitation: SFPQ Antibody [NBP2-20329] - Immunoprecipitation of SFPQ protein from 293T whole cell extracts using 5 ug of SFPQ antibody [N2C1], Internal. Western blot analysis was performed using SFPQ antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SFPQ Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SFPQ. The exact sequence is proprietary.
Localization
Nucleus matrix
Predicted Species
Rat (100%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFPQ
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:5000-1:20000
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-20329 in the following applications:

Reactivity Notes

Zebrafish (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for SFPQ Antibody

  • 100 kDa DNA-pairing protein
  • DNA-binding p52/p100 complex, 100 kDa subunit
  • hPOMp100
  • polypyrimidine tract binding protein associated
  • polypyrimidine tract-binding protein-associated splicing factor
  • Polypyrimidine tract-binding protein-associated-splicing factor
  • PSFPOMP100
  • PTB-associated splicing factor
  • PTB-associated-splicing factor
  • splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated)
  • splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated)
  • splicing factor proline/glutamine-rich
  • splicing factor, proline- and glutamine-rich

Background

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination. Alternate names for PSF/SFPQ include polypyrimidine tract-binding protein-associated-splicing factor, PTB-associated-splicing factor, splicing factor proline-and glutamine-rich, and POMP100.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SFPQ Antibody (NBP2-20329) (0)

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Review for SFPQ Antibody (NBP2-20329) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-20329:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Marina Villar
WB Human 06/19/2021
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Summary

ApplicationWestern Blot
Sample TestedHuman primary fibroblast
SpeciesHuman
Lot41927

Comments

CommentsIt works also in old membranes, even with fluorescent detection. Really good antibody.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SFPQ Antibody (NBP2-20329) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
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1
0

Marina Villar
06/19/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol SFPQ
Uniprot