![Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity. A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-22966-img0005.jpg "Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity. A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Pm, Eq, Pm, Pm-Or, Pm, RbSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human protein regulated in development and DNA damage response 1 (DNA-damage-inducible transcript 4) using the numbering given in entry NP_061931.1 (GeneID 54541). |
| Predicted Species | Rat (100%), Canine (100%), Primate (100%), Equine (100%), Monkey (100%), Bovine (100%), Orangutan (100%), Rabbit (100%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DDIT4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Formaldehyde fixation is recommended. Permeabilization with Triton X-100 is recommended for formaldehydefixed cells. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Natalie Erdmann |
ICC | Human | 05/24/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for DDIT4 Antibody (NBP1-22966)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - HeLa cells stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-22966-img0004.jpg "Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - HeLa cells stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight. Image from verified customer review.")
![Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-22966-img0002.jpg "Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.")
![Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity.(A-C) A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Western-Blot-NBP1-22966-img0006.jpg "Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity.(A-C) A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - NBF-fixed asynchronous, cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L crossadsorbed Antibody DyLight®594 used at 1:100.](http://images.novusbio.com/fullsize/DDIT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-22966-img0003.jpg "Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - NBF-fixed asynchronous, cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L crossadsorbed Antibody DyLight®594 used at 1:100.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
