![Western Blot: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLRE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLRE1B.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Western-Blot-NBP2-03563-img0004.jpg "Western Blot: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLRE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLRE1B.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2B7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.62 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-280 of human DCLRE1B(NP_073747) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DCLRE1B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 59.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1% BSA, 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.62 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Verified Customer |
WB | Human | 12/30/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCLRE1B .](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Immunocytochemistry-Immunofluorescence-NBP2-03563-img0009.jpg "Immunocytochemistry/Immunofluorescence: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCLRE1B .")
![Immunohistochemistry-Paraffin: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of paraffin-embedded Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Immunohistochemistry-Paraffin-NBP2-03563-img0007.jpg "Immunohistochemistry-Paraffin: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of paraffin-embedded Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.")
![Flow Cytometry: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DCLRE1B antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Flow-Cytometry-NBP2-03563-img0003.jpg "Flow Cytometry: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DCLRE1B antibody, and then analyzed by flow cytometry.")
![Western Blot: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - 1) 293T cell lysate. 2) 293T cell transfected to overexpress 3xFlag-DCLRE1B. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Western-Blot-NBP2-03563-img0008.jpg "Western Blot: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - 1) 293T cell lysate. 2) 293T cell transfected to overexpress 3xFlag-DCLRE1B. WB image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Immunohistochemistry-Paraffin-NBP2-03563-img0005.jpg "Immunohistochemistry-Paraffin: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DCLRE1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Immunohistochemistry-Paraffin-NBP2-03563-img0006.jpg "Immunohistochemistry-Paraffin: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DCLRE1B mouse monoclonal antibody.")
![Flow Cytometry: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Analysis of Hela cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Flow-Cytometry-NBP2-03563-img0001.jpg "Flow Cytometry: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Analysis of Hela cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Analysis of Jurkat cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Flow-Cytometry-NBP2-03563-img0002.jpg "Flow Cytometry: DCLRE1B Antibody (OTI2B7) [NBP2-03563] - Analysis of Jurkat cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![SDS-PAGE LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_719.jpg)
![Bioactivity LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_721.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
