![Western Blot: Artemis Antibody [NBP1-77975] - Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.](http://images.novusbio.com/fullsize/Artemis-Antibody-Western-Blot-NBP1-77975-img0002.jpg "Western Blot: Artemis Antibody [NBP1-77975] - Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Description | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified Artemis Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding an internal region of Human Artemis (DCLRE1C DNA cross-link repair 1C). (Uniprot: Q96SD1) |
| Specificity | This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express artemis, such as the cell line CJ (see figure) and in an hTERT line (not shown). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | DCLRE1C |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Reactivity in other immunoassays is unknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa). |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Artemis Antibody [NBP1-77975] - Used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Artemis-Antibody-Immunohistochemistry-NBP1-77975-img0003.jpg "Immunohistochemistry: Artemis Antibody [NBP1-77975] - Used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded.")
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