![Western Blot: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLRE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLRE1B.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Western-Blot-NBP2-72152-img0002.jpg "Western Blot: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLRE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLRE1B.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI2B7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-280 of human DCLRE1B(NP_073747) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DCLRE1B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 59.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DCLRE1B |
![Immunocytochemistry/Immunofluorescence: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCLRE1B .](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72152-img0006.jpg "Immunocytochemistry/Immunofluorescence: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCLRE1B .")
![Immunohistochemistry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DCLRE1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72152-img0008.jpg "Immunohistochemistry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DCLRE1B mouse monoclonal antibody.")
![Flow Cytometry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DCLRE1B antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72152-img0005.jpg "Flow Cytometry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DCLRE1B antibody, and then analyzed by flow cytometry.")
![Western Blot: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - 1) 293T cell lysate. 2) 293T cell transfected to overexpress 3xFlag-DCLRE1B. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Western-Blot-NBP2-72152-img0001.jpg "Western Blot: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - 1) 293T cell lysate. 2) 293T cell transfected to overexpress 3xFlag-DCLRE1B. WB image submitted by a verified customer review.")
![Immunohistochemistry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72152-img0007.jpg "Immunohistochemistry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.")
![Immunohistochemistry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of paraffin-embedded Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72152-img0009.jpg "Immunohistochemistry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Staining of paraffin-embedded Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody.")
![Flow Cytometry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Analysis of Hela cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72152-img0003.jpg "Flow Cytometry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Analysis of Hela cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Analysis of Jurkat cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DCLRE1B-Antibody-OTI2B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72152-img0004.jpg "Flow Cytometry: DCLRE1B Antibody (OTI2B7) - Azide and BSA Free [NBP2-72152] - Analysis of Jurkat cells, using anti-DCLRE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![SDS-PAGE LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_719.jpg)
![Bioactivity LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_721.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")