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MARCH8 Antibody

Images

 
Western Blot: MARCH8 Antibody [NBP1-85540] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: MARCH8 Antibody [NBP1-85540] - Staining of human Small intestine shows strong membranous and granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: MARCH8 Antibody [NBP1-85540] - Staining of human Cerebellum shows strong granular cytoplasmic positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: MARCH8 Antibody [NBP1-85540] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: MARCH8 Antibody [NBP1-85540] - Staining of human Testis shows strong membranous and granular cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MARCH8 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VQCKVYVQLWKRLKAYNRVIYVQNCPETSKKNIFEKSPLTEPNFENKHGHGICHSDTNSSCCTEPEDTGAEIIHV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MARCHF8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH6 retrieval is recommended.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
MARCH8 Protein (NBP1-85540PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MARCH8 Antibody

  • Cellular modulator of immune recognition
  • c-MIR
  • EC 6.3.2
  • EC 6.3.2.-
  • MARCH8
  • MARCH-VIII
  • MARCH-VIIIcellular modulator of immune recognition
  • membrane-associated ring finger (C3HC4) 8
  • Membrane-associated RING finger protein 8
  • Membrane-associated RING-CH protein VIII
  • MIR
  • RING finger protein 178
  • RNF178

Background

MARCH8 - membrane-associated ring finger (C3HC4) 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MARCHF8
Entrez
Uniprot