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CXCR5 Antibody (51505R) [Unconjugated]

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CXCR5 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190R) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
CXCR5 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190R) at 3 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
51505R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB190R-SP
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CXCR5 Antibody (51505R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302
Specificity
Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR5 Antibody (51505R) [Unconjugated]

  • BLR1
  • BLR1MDR-15
  • Burkitt lymphoma receptor 1
  • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
  • Burkitt lymphoma receptor 1, GTP-binding protein
  • CD185 antigen
  • CD185
  • chemokine (C-X-C motif) receptor 5
  • C-X-C chemokine receptor type 5
  • CXCR5
  • CXC-R5
  • MDR15CXCR-5
  • MGC117347
  • Monocyte-derived receptor 15

Background

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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