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CXCR4 Antibody (12G5) [Fluorescein]

 (12 Publications)
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Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB170F) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB170F) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # ...read more
Jurkat (filled histogram) vs K562 (open histogram) cells were stained with Mouse Anti-Human CXCR4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB170F). View our protocol for Staining ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
12G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB170U-100UG
Alexa Fluor 405
FAB170V-100UG
Alexa Fluor 488
FAB170G-025
Alexa Fluor 488
FAB170G-100
Alexa Fluor 532
FAB170X
Alexa Fluor 594
FAB170T-100UG
Alexa Fluor 647
FAB170R-100UG
Alexa Fluor 750
FAB170S-100UG
Allophycocyanin
FAB170A-025
Allophycocyanin
FAB170A-100
Allophycocyanin/Cy7
FAB170APCCY7
Biotin
FAB170B
DyLight 350
FAB170D
DyLight 405
FAB170E
DyLight 488
FAB170K
DyLight 550
FAB170L
DyLight 594
FAB170M
DyLight 650
FAB170W
DyLight 680
FAB170Y
DyLight 755
FAB170Z
Fluorescein
FAB170F-100
HRP
FAB170H
Janelia Fluor 549
FAB170I
Janelia Fluor 646
FAB170J
PE/Atto594
FAB170PEATT594
PE/Cy5.5
FAB170PECY55
PE/Cy7
FAB170PECY7
Phycoerythrin
FAB170P-100
Phycoerythrin
FAB170P-025
Unconjugated
MAB170
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB170
Carrier Free
FAB170G-025
Carrier Free
FAB170S-100UG
Carrier Free
FAB170T-100UG
Carrier Free
FAB170U-100UG
Carrier Free
FAB170V-100UG
Carrier Free
MAB170-SP
Carrier Free
MAB170
Carrier Free
FAB170G-100
Carrier Free
FAB170B
Carrier Free
FAB170A-100
Carrier Free
FAB170A-025
Carrier Free
FAB170P-100
Carrier Free
FAB170P-025
Carrier Free
FAB170F-100
Carrier Free
FAB170R-100UG
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CXCR4 Antibody (12G5) [Fluorescein] Summary

Immunogen
CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line
Accession # P61073
Specificity
Detects human CXCR4.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CXCR4
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB170F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (12G5) [Fluorescein]

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201E
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LAP-3
  • LCR1
  • LESTR
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3R
  • NPY3RWHIM
  • NPYR
  • NPYRL
  • NPYY3R
  • pCXCR4
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor
  • WHIMS

Background

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.

  1. Endres, M.J. et al. (1996) Cell 87:745.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCR4 Antibody
(12G5) [Fluorescein]
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CXCR4 Antibody (12G5) [Fluorescein]
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Publications for CXCR4 Antibody (FAB170F)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(10)
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Human
(10)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using FAB170F Applications Species
L Amniai, C Ple, M Barrier, P de Nadai, P Marquillie, H Vorng, C Chenivesse, A Tsicopoulo, C Duez Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine International Journal of Molecular Sciences, 2021-04-09;22(8):. 2021-04-09 [PMID: 33918621] (Flow Cytometry, Human) Flow Cytometry Human
Min Wang, Ru Feng, Jia-min Zhang, Lin-lin Xu, Fei-er Feng, Chen-cong Wang et al. Dysregulated megakaryocyte distribution associated with nestin+ mesenchymal stem cells in immune thrombocytopenia Blood Advances 5/14/2019 [PMID: 31053569]
Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human) Flow Cytometry Human
NINGQIANG MA, HAILIN PANG, WEIWEI SHEN, FENG ZHANG, ZAOXUN CUI, JUNYAN WANG et al. Downregulation of CXCR4 by SDF-KDEL in SBC-5 cells inhibits their migration in vitro and organ metastasis in vivo International Journal of Molecular Medicine 2/1/2015 [PMID: 25504108]
Li Q, Zhang A, Tao C, Li X, Jin P The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro. Biochem Biophys Res Commun, 2013-10-30;441(3):675-80. 2013-10-30 [PMID: 24184476] (Flow Cytometry, Human) Flow Cytometry Human
Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011-10-21;6(10):e26780. 2011-10-21 [PMID: 22039549] (Flow Cytometry, Human) Flow Cytometry Human
Bosco MC, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, Eva A, Novelli F, Cappello P, Giovarelli M, Varesio L Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo. Blood, 2010-12-10;117(9):2625-39. 2010-12-10 [PMID: 21148811] (Flow Cytometry, Human) Flow Cytometry Human
Hajishengallis G, Wang M, Liang S, Triantafilou M, Triantafilou K Pathogen induction of CXCR4/TLR2 cross-talk impairs host defense function. Proc. Natl. Acad. Sci. U.S.A., 2008-09-02;105(36):13532-7. 2008-09-02 [PMID: 18765807] (Flow Cytometry, Human) Flow Cytometry Human
Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis. Allergy, 2007-02-01;62(2):170-7. 2007-02-01 [PMID: 17298426] (Flow Cytometry, Human) Flow Cytometry Human
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Flow Cytometry, Human) Flow Cytometry Human
Show All 12 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCR4 Antibody (FAB170F). (Showing 1 - 1 of 1 FAQs).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Secondary Antibodies

 

Isotype Controls

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Blogs on CXCR4.

Check out the latest blog posts on CXCR4.
Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.
LAMP2: Protector of the lysosome
LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.
CXCR4 Studies on Neural and Stem Cells
The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.
Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB170P) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) for 15 minutes at 37°C followed by 15 minutes at room temperature. View our protocol for Staining Membrane-associated Proteins.
CXCR4 Antibody (12G5) [Phycoerythrin]
FAB170P-025
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Bioinformatics

Gene Symbol CXCR4
Uniprot