Home » CXCL13/BLC/BCA-1 » CXCL13 Antibodies » CXCL13/BLC/BCA-1 Antibody (143614) [Unconjugated]
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CXCL13/BLC/BCA-1 Antibody (143614) [Unconjugated]

 (13 Publications)
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Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA‑1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL13/BLC/BCA‑1 Monoclonal Antibody (Catalog # MAB470). The ND50 is typically 12-30 µg/mL.
Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
143614
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB470-SP
Carrier Free
MAB470-500
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CXCL13/BLC/BCA-1 Antibody (143614) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CXCL13
Ile22-Ala109
Accession # O55038
Specificity
Detects mouse CXCL13 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL13, rmCXCL11/I‑TAC, rmCXCL9/MIG, or rm CXCL12/SDF‑1 alpha .
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CXCL13
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 12-30 ug/mL
Application Notes
ELISA Detection: Mouse CXCL13/BLC/BCA-1 Biotinylated Antibody (Catalog number BAF470)
Standard: Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog number 470-BC)
Publications
Read Publications using
MAB470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL13/BLC/BCA-1 Antibody (143614) [Unconjugated]

  • ANGIE
  • ANGIE2
  • B cell-attracting chemokine 1
  • B lymphocyte chemoattractant
  • BCA1
  • BCA-1
  • BCA1B-cell chemoattractant
  • BCA-1CXC chemokine BLC
  • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
  • BLC
  • BLCSmall-inducible cytokine B13
  • BLR1L
  • B-lymphocyte chemoattractant
  • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
  • chemokine (C-X-C motif) ligand 13
  • C-X-C motif chemokine 13
  • CXCL13
  • SCYB13
  • SCYB13B-cell-attracting chemokine 1
  • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC) and BCA-1, is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse CXCL13 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse CXCL13 shares 64% amino acid sequence similarity with the human protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized CXCL13 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for CXCL13. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCL13/BLC/BCA-1
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CXCL13/BLC/BCA-1 Antibody (143614) [Unconjugated]
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Publications for CXCL13/BLC/BCA-1 Antibody (MAB470)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Cell Selection, Direct ELISA, IHC-Fr, Luminex Development, Neutralization.


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Cell Selection
(1)
Direct ELISA
(1)
IHC-Fr
(1)
Luminex Development
(1)
Neutralization
(4)
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Mouse
(8)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB470 Applications Species
KW Ng, J Boumelha, KSS Enfield, J Almagro, H Cha, O Pich, T Karasaki, DA Moore, R Salgado, M Sivakumar, G Young, M Molina-Arc, S de Carné T, P Anastasiou, A Fendler, L Au, STC Shepherd, C Martínez-R, C Puttick, JRM Black, TBK Watkins, H Kim, S Shim, N Faulkner, J Attig, S Veeriah, N Magno, S Ward, AM Frankell, M Al Bakir, EL Lim, MS Hill, GA Wilson, DE Cook, NJ Birkbak, A Behrens, N Yousaf, S Popat, A Hackshaw, TRACERx Co, CAPTURE Co, CT Hiley, K Litchfield, N McGranahan, M Jamal-Hanj, J Larkin, SH Lee, S Turajlic, C Swanton, J Downward, G Kassiotis Antibodies against endogenous retroviruses promote lung cancer immunotherapy Nature, 2023-04-12;616(7957):563-573. 2023-04-12 [PMID: 37046094] (Neutralization, Mouse) Neutralization Mouse
Shubhra Singh, Jason Roszik, Neeraj Saini, Vipul Kumar Singh, Karishma Bavisi, Zhiqiang Wang et al. B Cells Are Required to Generate Optimal Anti-Melanoma Immunity in Response to Checkpoint Blockade Frontiers in Immunology 2022-05-26 [PMID: 35720386]
Chaurio RA, Anadon CM, Lee Costich T et al. TGF-?-mediated silencing of genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures Immunity 2022-01-01 [PMID: 35021053]
Emma S. Crichton, Shan Zeng, G. Michael La Muraglia, I. Raul Badell CXCL13 Is an Indicator of Germinal Center Activity and Alloantibody Formation Following Transplantation Transplantation Direct 2021-11-05 [PMID: 34778545]
Ma L, Yu L, Jiang BC Et al. ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons The Journal of experimental medicine 12/6/2021 [PMID: 34762123]
Klimatcheva E, Pandina T, Reilly C, Torno S, Bussler H, Scrivens M, Jonason A, Mallow C, Doherty M, Paris M, Smith E, Zauderer M CXCL13 antibody for the treatment of autoimmune disorders. BMC Immunol, 2015-02-12;16(0):6. 2015-02-12 [PMID: 25879435] (Direct ELISA, Mouse) Direct ELISA Mouse
Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections. Am J Pathol, 2014-12-06;185(2):420-31. 2014-12-06 [PMID: 25485859] (Cell Selection, Mouse) Cell Selection Mouse
Kramer J, Klimatcheva E, Rothstein T CXCL13 is elevated in Sjogren&#039;s syndrome in mice and humans and is implicated in disease pathogenesis. J Leukoc Biol, 2013-07-31;94(5):1079-89. 2013-07-31 [PMID: 23904442] (Neutralization, Mouse) Neutralization Mouse
Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage. J. Immunol., 2012-07-27;189(5):2181-90. 2012-07-27 [PMID: 22844119] (IHC-Fr, Mouse) IHC-Fr Mouse
Leon B, Ballesteros-Tato A, Browning JL, Dunn R, Randall TD, Lund FE Regulation of T(H)2 development by CXCR5+ dendritic cells and lymphotoxin-expressing B cells. Nat. Immunol., 2012-05-27;13(7):681-90. 2012-05-27 [PMID: 22634865] (Neutralization, Mouse) Neutralization Mouse
Show All 13 Publications.

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Bioinformatics

Gene Symbol CXCL13
Uniprot