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CX3CL1/Fractalkine Antibody [Unconjugated]

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Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
Cardiomyocyte-specific ADAM10/CX3CL1 signaling regulates neutrophil migration.a Immunofluorescence images and b quantification of CX3CL1, cardiac Troponin T (cTnT, cardiomyocytes, CM) and CD31 (endothelial cells, EC) ...read more
Cardiomyocyte-specific ADAM10/CX3CL1 signaling regulates neutrophil migration.a Immunofluorescence images and b quantification of CX3CL1, cardiac Troponin T (cTnT, cardiomyocytes, CM) and CD31 (endothelial cells, EC) ...read more
Cardiomyocyte-specific ADAM10 KO improves post-MI cardiac function.a–c Echocardiographic assessment of fractional area shortening, ejection fraction and left ventricular end-systolic interior diameter in 8-week-old ...read more
Cardiomyocyte-specific ADAM10/CX3CL1 signaling regulates neutrophil migration.a Immunofluorescence images and b quantification of CX3CL1, cardiac Troponin T (cTnT, cardiomyocytes, CM) and CD31 (endothelial cells, EC) ...read more
Cardiomyocyte-specific ADAM10/CX3CL1 signaling regulates neutrophil migration.a Immunofluorescence images and b quantification of CX3CL1, cardiac Troponin T (cTnT, cardiomyocytes, CM) and CD31 (endothelial cells, EC) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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CX3CL1/Fractalkine Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CX3CL1/Fractalkine
Leu22-Lys105
Accession # O35188
Specificity
Detects mouse Fractalkine in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human Fractalkine and recombinant rat Fractalkine is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CX3CL1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.3-1.5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF472 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody [Unconjugated]

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CX3CL1/Fractalkine Antibody (AF472)(18)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 8 applications: Bioassay, Flow Cytometry, ICC, IHC, IHC-P, In Vivo, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(2)
ICC
(2)
IHC
(4)
IHC-P
(3)
In Vivo
(1)
Neutralization
(2)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(13)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF472 Applications Species
Long, J;Liu, H;Qiu, Z;Xiao, Z;Lu, Z; Glabridin Therapy Reduces Chronic Allodynia, Spinal Microgliosis, and Dendritic Spine Generation by Inhibiting Fractalkine-CX3CR1 Signaling in a Mouse Model of Tibial Fractures Brain sciences 2023-04-29 [PMID: 37239211] (In Vivo, Mouse) In Vivo Mouse
E Klapproth, A Witt, P Klose, J Wiedemann, N Vavilthota, SR Künzel, S Kämmerer, M Günscht, D Sprott, M Lesche, F Rost, A Dahl, E Rauch, L Kattner, S Weber, P Mirtschink, I Kopaliani, K Guan, K Lorenz, P Saftig, M Wagner, A El-Armouch Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction Nature Communications, 2022-12-10;13(1):7648. 2022-12-10 [PMID: 36496449] (Western Blot, Mouse) Western Blot Mouse
Y Sun, XX Zhang, S Huang, H Pan, YZ Gai, YQ Zhou, L Zhu, HZ Nie, DX Li Diet-Induced Obesity Promotes Liver Metastasis of Pancreatic Ductal Adenocarcinoma via CX3CL1/CX3CR1 Axis Journal of Immunology Research, 2022-04-18;2022(0):5665964. 2022-04-18 [PMID: 35478937] (Bioassay, Mouse) Bioassay Mouse
Cooper A. Brett, Julianne B. Carroll, Mark L. Gabriele Compromised fractalkine signaling delays microglial occupancy of emerging modules in the multisensory midbrain Glia 2022-04-01 [PMID: 35132709]
Sergio M. Borghi, Victor Fattori, Thacyana T. Carvalho, Vera L. H. Tatakihara, Tiago H. Zaninelli, Felipe A. Pinho-Ribeiro et al. Experimental Trypanosoma cruzi Infection Induces Pain in Mice Dependent on Early Spinal Cord Glial Cells and NF kappa B Activation and Cytokine Production Frontiers in Immunology 2021-01-26 [PMID: 33574810]
Sergio M. Borghi, Victor Fattori, Felipe A. Pinho-Ribeiro, Talita P. Domiciano, Milena M. Miranda-Sapla, Tiago H. Zaninelli et al. Contribution of spinal cord glial cells to L. amazonensis experimental infection-induced pain in BALB/c mice Journal of Neuroinflammation 2019-12-01 [PMID: 31138231]
Jingjing Zhang, Yufei Liu, Xinyao Liu, Song Li, Cheng Cheng, Sheng Chen et al. Dynamic changes of CX3CL1/CX3CR1 axis during microglial activation and motor neuron loss in the spinal cord of ALS mouse model Translational Neurodegeneration 2018-12-21 [PMID: 30607245]
M Bolós, M Llorens-Ma, JR Perea, J Jurado-Arj, A Rábano, F Hernández, J Avila Absence of CX3CR1 impairs the internalization of Tau by microglia Mol Neurodegener, 2017-08-15;12(1):59. 2017-08-15 [PMID: 28810892] (IHC, Mouse) IHC Mouse
Bryan Simons PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen J. Immunol, 2016-11-28;0(0):. 2016-11-28 [PMID: 27895167] (IHC-P, Mouse) IHC-P Mouse
Yoh Goto CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow Stem Cells, 2016-07-08;0(0):. 2016-07-08 [PMID: 27339271] (Neutralization, Mouse) Neutralization Mouse
Show All 18 Publications.

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Bioinformatics

Gene Symbol CX3CL1
Uniprot