![Western Blot: CRISP-2 Antibody [NBP1-52012] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B.](http://images.novusbio.com/fullsize/CRISP-2-Antibody-Western-Blot-NBP1-52012-img0003.jpg "Western Blot: CRISP-2 Antibody [NBP1-52012] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B.")
| Reactivity | Hu, Po, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QHSDPEDRKTSTR corresponding to internal region according to NP_003287.1. |
| Epitope | C-QHSDPEDRKTSTR |
| Specificity | Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CRISP2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Primary incubations were for 1 hour.
Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide IHC: Paraffin embedded Human Testis. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-52012 | Applications | Species |
|---|---|---|
| Jamsai D, Reilly A, Smith SJ et al. Polymorphisms in the human cysteine-rich secretory protein 2 (CRISP2) gene in Australian men. Hum Reprod 2008-09-01 [PMID: 18550510] |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CRISP2 |
| Entrez |
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| Uniprot |
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![Immunohistochemistry-Paraffin: CRISP-2 Antibody [NBP1-52012] - 3.75ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CRISP-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52012-img0004.jpg "Immunohistochemistry-Paraffin: CRISP-2 Antibody [NBP1-52012] - 3.75ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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