![Western Blot: Collagen V Antibody (1E2-E4/Col5) [NBP1-05118] - Staining with 1E2-E4/Col5 on Dog Type I, III, V and Human Type I, III, and V in rows 1-6 respectively. (1. Werkmeister)](http://images.novusbio.com/fullsize/Collagen-V-Antibody-1E2-E4-Col5-Western-Blot-NBP1-05118-img0001.jpg "Western Blot: Collagen V Antibody (1E2-E4/Col5) [NBP1-05118] - Staining with 1E2-E4/Col5 on Dog Type I, III, V and Human Type I, III, and V in rows 1-6 respectively. (1. Werkmeister)")
| Reactivity | Hu, Po, Bv, Ca, Ma, Rb, Sh, Ch(-), Gp(-), Mu(-), Rt(-)Species Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 1E2-E4/Col5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Acid-digested pepsin soluble dog type V collagen |
| Specificity | Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I,
III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other
connective tissue proteins (laminin, fibronectin, elastin). |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | COL5A1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | ELISA: NBP1-05118 can be used for detection of collagens by ELISA. NBP1-05118 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
WB: In immunoblotting NBP1-05118 detects human type V collagen only in its native triple helix form.
IHC: NBP1-05118 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended. |
|
| Publications |
|
| Storage | Store at 4C in the dark. |
| Buffer | 10mM Phosphate (pH 7.4) and 0.5M NaCl |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
![Simple Western Decorin Antibody [Unconjugated]](https://images.novusbio.com/images2/Decorin_AF1060_Simple_Western_18969.jpg)
![Western Blot Decorin Antibody [Unconjugated]](https://images.novusbio.com/images2/Decorin_AF1060_Western_Blot_19006.jpg)
![Immunohistochemistry Decorin Antibody [Unconjugated]](https://images.novusbio.com/images/af1060_mouse-decorin-affinity-purified-polyclonal-ab-8120255552891.jpg)
| Publications using NBP1-05118 | Applications | Species |
|---|---|---|
| Velez CL. Integrinas y su regulacion por el sistema inmune durante la gestacion porcina. Thesis. 2017-01-01 (Porcine) | Porcine | |
| Werkmeister J Ramshaw J. Monoclonal antibodies to Type V Collagen for Immunohistological Examination of New Tissue Deposition Associated with Biomaterial Implants. J Histochemistry and Cytochemistry. 1991-01-01 [PMID: 1918939] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Collagen V Antibody (NBP1-05118)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | COL5A1 |
![Western Blot Tenascin X Antibody [Unconjugated]](https://images.novusbio.com/images2/Tenascin_X_AF6999_Western_Blot_11365.jpg)
![Immunohistochemistry Tenascin X Antibody [Unconjugated]](https://images.novusbio.com/images2/Tenascin_X_AF6999_Immunohistochemistry_11545.jpg)
![Simple Western Tenascin X Antibody [Unconjugated]](https://images.novusbio.com/images2/Tenascin_X_AF6999_Simple_Western_20232.jpg)
