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Coagulation Factor III/Tissue Factor Antibody [Unconjugated]

 (14 Publications)
 (2 Reviews)
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Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Coagulation Factor III/Tissue Factor at approximately 48 kDa (as indicated). GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Coagulation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Biotin
BAF2339
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Carrier Free
AF2339-SP
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Coagulation Factor III/Tissue Factor Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor
Gly34-Glu251
Accession # P13726
Specificity
Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
F3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2339 in the following applications:
Publications
Read Publications using
AF2339 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody [Unconjugated]

  • CD142 antigen
  • CD142
  • coagulation factor III (thromboplastin, tissue factor)
  • Coagulation Factor III
  • F3
  • FLJ17960
  • TF
  • TFA
  • Thromboplastin
  • Tissue Factor

Background

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).

  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
  3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
  4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
  5. Morrissey, J.H. et al. (1987) Cell 50:129.
  6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Coagulation Factor III/Tissue Factor Antibody (AF2339)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Development, Flow Cytometry, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF2339 Applications Species
Ana T.A. Sachetto, Sierra J. Archibald, Ravi Bhatia, Dougald Monroe, Yohei Hisada, Nigel Mackman Evaluation of four commercial ELISAs to measure tissue factor in human plasma Research and Practice in Thrombosis and Haemostasis 2023-03-28 [PMID: 37275179]
C Mwase, TN Phung, MJ O'Sullivan, JA Mitchel, M De Marzio, A K?l?ç, ST Weiss, JJ Fredberg, JA Park Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C Cells, 2022-01-13;11(2):. 2022-01-13 [PMID: 35053372] (Western Blot, Human) Western Blot Human
TLR Humphries, K Shen, A Iyer, DW Johnson, GC Gobe, D Nikolic-Pa, DP Fairlie, DA Vesey PAR2-Induced Tissue Factor Synthesis by Primary Cultures of Human Kidney Tubular Epithelial Cells Is Modified by Glucose Availability International Journal of Molecular Sciences, 2021-07-14;22(14):. 2021-07-14 [PMID: 34299151] (Western Blot, Human) Western Blot Human
A Iyer, TLR Humphries, EP Owens, KN Zhao, PP Masci, DW Johnson, D Nikolic-Pa, GC Gobe, DP Fairlie, DA Vesey PAR2 Activation on Human Kidney Tubular Epithelial Cells Induces Tissue Factor Synthesis, That Enhances Blood Clotting Frontiers in Physiology, 2021-03-10;12(0):615428. 2021-03-10 [PMID: 33776786] (Western Blot, Human) Western Blot Human
Izmarie Poventud‐Fuentes, Keon Woo Kwon, Jeongyun Seo, Maurizio Tomaiuolo, Timothy J. Stalker, Lawrence F. Brass et al. A Human Vascular Injury‐on‐a‐Chip Model of Hemostasis Small 2021-04-01 [PMID: 33150735]
Desirée Edén, Grigorios Panagiotou, Dariush Mokhtari, Jan W. Eriksson, Mikael Åberg, Agneta Siegbahn Adipocytes express tissue factor and FVII and are procoagulant in a TF/FVIIa-dependent manner Upsala Journal of Medical Sciences 2019-08-14 [PMID: 31407948]
Åsa Thulin, Junhong Yan, Mikael Åberg, Christina Christersson, Masood Kamali-Moghaddam, Agneta Siegbahn Sensitive and Specific Detection of Platelet-Derived and Tissue Factor–Positive Extracellular Vesicles in Plasma Using Solid-Phase Proximity Ligation Assay TH Open 2018-07-27 [PMID: 31276087]
LM Gockel, JM Ponert, S Schwarz, M Schlesinge, G Bendas The Low Molecular Weight Heparin Tinzaparin Attenuates Platelet Activation in Terms of Metastatic Niche Formation by Coagulation-Dependent and Independent Pathways Molecules, 2018-10-24;23(11):. 2018-10-24 [PMID: 30356007] (Flow Cytometry, Human) Flow Cytometry Human
Kinchen J, Chen HH, Parikh K et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell 9/25/2018 [PMID: 30270042]
Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 2016-05-04;6(0):25171. 2016-05-04 [PMID: 27142573] (IHC-P, Human) IHC-P Human
Show All 14 Publications.

Reviews for Coagulation Factor III/Tissue Factor Antibody (AF2339) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2339:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22828416
SpeciesHuman
  3
reviewed by:
Verified Customer
 IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23468018
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Coagulation Factor III/Tissue Factor Antibody (AF2339). (Showing 1 - 1 of 1 FAQs).

  1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
    • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Secondary Antibodies

 

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Recent Reviews

4
5
1
4
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3
1
2
0
1
0
 
 Verified Customer
01/06/2015
Application: WB
Species: Human
read this review
 
 Verified Customer
01/06/2015
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol F3
Uniprot