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TSTA3 Antibody

Images

 
Western Blot: TSTA3 Antibody [NBP1-83408] - Analysis in control (vector only transfected HEK293T lysate) and TSTA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TSTA3 Antibody [NBP1-83408] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TSTA3 Antibody [NBP1-83408] - Staining of human testis shows distinct nuclear and cytoplasmic positivity in Leydig cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TSTA3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RILVTGGSGLVGKAIQKVVADGAGLPGEDWVFVSSKDADLTDTAQTRALFEKVQPTHVIHLAAMVG
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSTA3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TSTA3 Protein (NBP1-83408PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TSTA3 Antibody

  • EC 1.1.1.271
  • FX
  • GDP-4-keto-6-deoxy-D-mannose epimerase-reductase
  • GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
  • GDP-L-fucose synthase
  • P35B
  • Protein FX
  • Red cell NADP(H)-binding protein
  • SDR4E13-5 epimerase/4-reductase
  • short chain dehydrogenase/reductase family 4E, member 1
  • Short-chain dehydrogenase/reductase family 4E member 1
  • tissue specific transplantation antigen 3
  • tissue specific transplantation antigen P35B
  • Tissue-specific transplantation antigen-3

Background

Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TSTA3