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Coagulation Factor III/Tissue Factor Antibody (CL3807)

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Genetic Strategies: Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, ...read more
Western Blot: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-431
Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
Immunohistochemistry: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human colon shows strong immunoreactivity in pericryptal sheath cells.
Immunohistochemistry: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human kidney shows strong positivity in renal glomerulus.
Immunohistochemistry: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human placenta shows strong immunoreactivity in blood vessels.
Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human testis shows strong positivity in the lamina propria.
Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody (CL3807) [NBP2-61641] - Staining of human lung cancer shows strong immunoreactivity in a subset of cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KD
Clone
CL3807
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Coagulation Factor III/Tissue Factor Antibody (CL3807) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: VKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGEN
Epitope
SPEFTPYLET
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
F3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Coagulation Factor III/Tissue Factor Antibody (CL3807)

  • CD142 antigen
  • CD142
  • coagulation factor III (thromboplastin, tissue factor)
  • Coagulation Factor III
  • F3
  • FLJ17960
  • TF
  • TFA
  • Thromboplastin
  • Tissue Factor

Background

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Coagulation Factor III/Tissue Factor Antibody (NBP2-61641). (Showing 1 - 1 of 1 FAQ).

  1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
    • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol F3