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cIAP-1/HIAP-2 Antibody [Unconjugated]

Name: cIAP-1/HIAP-2 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF8181
Rating: 4 (4 reviews)

(66 Publications)

(4 Reviews)

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Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 (lane 1), human cIAP-2 (lane 2), or non-transfected (lane 3). PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human ...read more

cIAP-1/HIAP-2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 10 µg/mL overnight at ...read more

cIAP-1/HIAP-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 15 µg/mL overnight at 4 ...read more

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) using 5 ...read more

Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat ...read more

Genetic Strategies: Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP‑1/HIAP‑2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific ...read more

IAP expression in glioblastoma cell lines. Expression levels of cIAP1, cIAP2, XIAP and ML-IAP were analyzed by western blotting and quantified in U87MG and GL261 adherent GBM cell lines, and in GBM6 and GBM9 spheres. ...read more

cIAP-1 and cIAP-2 are downregulated in cholangiocytes of PSC patients. (a) Representative images of liver sections stained for cIAP-1 (left panel) and cIAP-2 (right panel) in patients with normal, NASH and PSC (stage ...read more

Prognostic value of cIAP1, cIAP2, XIAP and ML-IAP protein expression in human glioblastomas (cohorts 1 and 2). (a) cIAP1-, cIAP2-, XIAP- and ML-IAP-positive stainings in GBM. IAPs were heterogeneously expressed by tumor ...read more

Apoptosis and IAP expression upon SMAC mimetic GDC-0152 treatment in glioblastoma cell lines. (a) Apoptosis (SubG0/G1) of DMSO control and GDC-0152-treated cells was determined by flow cytometry of propidium ...read more

BV6 considerably reduces expression of IAP proteins. SW480, HT-29 and HCT-15 cells were plated in a 3D laminin-rich extracellular matrix and treated with 1 μM BV6 or DMSO control 4 h before irradiation with 4 Gy. At ...read more

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB, Simple Western, IHC, KO
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Validated by:

Genetic Strategies

Order Details

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Catalog# & Formulation	Size	Price
Carrier Free AF8181-SP

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cIAP-1/HIAP-2 Antibody [Unconjugated] Summary

Immunogen	E. coli-derived recombinant human cIAP-1/HIAP-2 His2-Ser618 Accession # Q13490
Specificity	Detects human cIAP-1/HIAP-2. Does not cross-react with recombinant human cIAP-2 or XIAP.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	BIRC2
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunohistochemistry 5-15 ug/mL
Knockout Validated
Simple Western 5 ug/mL
Western Blot 0.5 ug/mL

Application Notes

In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Reviewed Applications

Read 4 Reviews rated 4

using
AF8181 in the following applications:

Western Blot

Publications

Read Publications using
AF8181 in the following applications:

Flow Cytometry
2 publications
ICC
1 publication
IHC
3 publications
Immunoprecipitation
5 publications
Proximity Ligation Assay (PLA)
1 publication
Western Blot
42 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cIAP-1/HIAP-2 Antibody [Unconjugated]

API1Hiap-2
apoptosis inhibitor 1
baculoviral IAP repeat containing 2
baculoviral IAP repeat-containing 2
baculoviral IAP repeat-containing protein 2
BIRC2
cIAP1
c-IAP1
cIAP-1
hIAP2
HIAP-2
IAP homolog B
IAP2
IAP-2
Inhibitor of apoptosis protein 2
MIHB
MIHBHIAP2
NFR2-TRAF signalling complex protein
RING finger protein 48
RNF48hiap-2
TNFR2-TRAF-signaling complex protein 2

Background

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta .

Roy, N. et al. (1997) EMBO J. 23:6914.
Deveraux, Q. et al. (1997) Nature 388:300.
Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
Herrera, B. et al. (2002) FEBS Letters 520:93.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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cIAP-1/HIAP-2
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Publications for cIAP-1/HIAP-2 Antibody (AF8181)(66)

We have publications tested in 3 confirmed species: Human, Mouse, Feline.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot.

Filter By Application

Flow Cytometry

(2)

ICC

(1)

IHC

(3)

Immunoprecipitation

(5)

Proximity Ligation Assay (PLA)

(1)

Western Blot

(42)

All Applications

Filter By Species

Human

(45)

Mouse

(1)

Feline

(1)

All Species

Showing Publications 1 - 10 of 66. Show All 66 Publications.

Publications using AF8181	Applications	Species
Tencer, AH;Yu, Y;Causse, SZ;Campbell, GR;Klein, BJ;Xuan, H;Cartier, J;Miles, MA;Gaurav, N;Zadoroznyj, A;Holt, TA;Wen, H;Hawkins, CJ;Spector, SA;Dubrez, L;Shi, X;Kutateladze, TG; Molecular basis for nuclear accumulation and targeting of the inhibitor of apoptosis BIRC2 Nature structural & molecular biology 2023-07-31 [PMID: 37524969] (Western Blot, Human)	Western Blot	Human
EL Orth-He, HC Huang, SD Rao, Q Wang, Q Chen, CM O'Mara, AJ Chui, M Saoi, AR Griswold, A Bhattachar, DP Ball, JR Cross, DA Bachovchin Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation Cell Reports, 2023-01-16;0(0):111965. 2023-01-16 [PMID: 36649711] (Western Blot, Human)	Western Blot	Human
B Dumétier, A Zadoroznyj, J Berthelet, S Causse, J Allègre, P Bourgeois, F Cattin, C Racoeur, C Paul, C Garrido, L Dubrez cIAP1/TRAF2 interplay promotes tumor growth through the activation of STAT3 Oncogene, 2022-11-18;0(0):. 2022-11-18 [PMID: 36400972] (Western Blot, Human)	Western Blot	Human
Y Akizuki, M Morita, Y Mori, A Kaiho-Soma, S Dixit, A Endo, M Shimogawa, G Hayashi, M Naito, A Okamoto, K Tanaka, Y Saeki, F Ohtake cIAP1-based degraders induce degradation via branched ubiquitin architectures Nature Chemical Biology, 2022-10-31;0(0):. 2022-10-31 [PMID: 36316570] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Christian Holmgren, Ellen Sunström Thörnberg, Victoria Granqvist, Christer Larsson Induction of Breast Cancer Cell Apoptosis by TRAIL and Smac Mimetics: Involvement of RIP1 and cFLIP Current Issues in Molecular Biology 10/11/2022 [PMID: 36286042]
Hongli Yin, Ozge Karayel, Ying-Yin Chao, Thomas Seeholzer, Isabel Hamp, Oliver Plettenburg et al. A20 and ABIN-1 cooperate in balancing CBM complex-triggered NF-kappa B signaling in activated T cells Cellular and Molecular Life Sciences 2/1/2022 [PMID: 35099607]
V Granqvist, C Holmgren, C Larsson Induction of interferon-&beta and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells PLoS ONE, 2021-03-26;16(3):e0248175. 2021-03-26 [PMID: 33770100] (Western Blot, Human)	Western Blot	Human
Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas Scientific Reports, 2021-03-18;11(1):6392. 2021-03-18 [PMID: 33737574] (Western Blot, Human)	Western Blot	Human
Zhijun Liu, Himani Nailwal, Jonah Rector, Masmudur M. Rahman, Richard Sam, Grant McFadden et al. A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation Immunity 2/1/2021 [PMID: 33444549]
Lars Pache, Matthew D. Marsden, Peter Teriete, Alex J. Portillo, Dominik Heimann, Jocelyn T. Kim et al. Pharmacological Activation of Non-canonical NF-kappa B Signaling Activates Latent HIV-1 Reservoirs In Vivo Cell Reports Medicine 6/23/2020 [PMID: 33205060]
DE Muench, A Olsson, K Ferchen, G Pham, RA Serafin, S Chutipongt, P Dwivedi, B Song, S Hay, K Chetal, LR Trump-Durb, J Mookerjee-, K Zhang, JC Yu, C Lutzko, KC Myers, KL Nazor, KD Greis, DJ Kappes, SS Way, N Salomonis, HL Grimes Mouse models of neutropenia reveal progenitor-stage-specific defects Nature, 2020-04-22;582(7810):109-114. 2020-04-22 [PMID: 32494068] (IHC, Human)	IHC	Human
CC Nixon, M Mavigner, GC Sampey, AD Brooks, RA Spagnuolo, DM Irlbeck, C Mattingly, PT Ho, N Schoof, CG Cammon, GK Tharp, M Kanke, Z Wang, RA Cleary, AA Upadhyay, C De, SR Wills, SD Falcinelli, C Galardi, H Walum, NJ Schramm, J Deutsch, JD Lifson, CM Fennessey, BF Keele, S Jean, S Maguire, B Liao, EP Browne, RG Ferris, JH Brehm, D Favre, TH Vanderford, SE Bosinger, CD Jones, JP Routy, NM Archin, DM Margolis, A Wahl, RM Dunham, G Silvestri, A Chahroudi, JV Garcia Systemic HIV and SIV latency reversal via non-canonical NF-kappaB signalling in vivo Nature, 2020-01-22;578(7793):160-165. 2020-01-22 [PMID: 31969707] (IHC, Human)	IHC	Human
J Dittmann, T Haydn, P Metzger, GA Ward, M Boerries, M Vogler, S Fulda Next-generation hypomethylating agent SGI-110 primes acute myeloid leukemia cells to IAP antagonist by activating extrinsic and intrinsic apoptosis pathways Cell Death Differ., 2019-12-12;0(0):. 2019-12-12 [PMID: 31831875] (Western Blot, Human)	Western Blot	Human
LGT Lemos, GMDC Longo, BDS Mendonça, MC Robaina, MCM Brum, CA Cirilo, ERP Gimba, PRR Costa, CD Buarque, G Nestal de, RC Maia The LQB-223 Compound Modulates Antiapoptotic Proteins and Impairs Breast Cancer Cell Growth and Migration Int J Mol Sci, 2019-10-12;20(20):. 2019-10-12 [PMID: 31614718] (Western Blot, Human)	Western Blot	Human
Roy Xiao, Yi An, Wenda Ye, Adeeb Derakhshan, Hui Cheng, Xinping Yang et al. Dual Antagonist of cIAP/XIAP ASTX660 Sensitizes HPV(−) and HPV(+) Head and Neck Cancers To TNF alpha , TRAIL, and Radiation Therapy Clinical Cancer Research 11/1/2019 [PMID: 31266830]
Yoonseon Jeong, Joo Weon Lim, Hyeyoung Kim Lycopene Inhibits Reactive Oxygen Species-Mediated NF-kappa B Signaling and Induces Apoptosis in Pancreatic Cancer Cells Nutrients 4/1/2019 [PMID: 30939781]
S Lee, JY Lee, EW Lee, S Park, DH Kang, C Min, DJ Lee, D Kang, J Song, J Kwon, SW Kang Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes Cell Rep, 2019-02-19;26(8):2194-2211.e6. 2019-02-19 [PMID: 30784599] (Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot, Human)	Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot	Human
Nobumichi Ohoka, Osamu Ujikawa, Kenichiro Shimokawa, Tomoya Sameshima, Norihito Shibata, Takayuki Hattori et al. Different Degradation Mechanisms of Inhibitor of Apoptosis Proteins (IAPs) by the Specific and Nongenetic IAP-Dependent Protein Eraser (SNIPER) Chemical and Pharmaceutical Bulletin 3/1/2019 [PMID: 30369550]
J Allègre, J Cartier, V Glorian, N Droin, B Dumetier, C Kayaci, J Berthelet, S Gemble, C Vuillier, L Maillet, C Garrido, L Dubrez E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding PLoS ONE, 2018-10-25;13(10):e0206253. 2018-10-25 [PMID: 30359437] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
N Shibata, K Shimokawa, K Nagai, N Ohoka, T Hattori, N Miyamoto, O Ujikawa, T Sameshima, H Nara, N Cho, M Naito Pharmacological difference between degrader and inhibitor against oncogenic BCR-ABL kinase Sci Rep, 2018-09-10;8(1):13549. 2018-09-10 [PMID: 30202081] (Western Blot, Human)	Western Blot	Human
Yuko Fujiwara, Motonobu Saito, Ana I. Robles, Momoyo Nishida, Fumitaka Takeshita, Masatoshi Watanabe et al. A Nucleolar Stress–Specific p53–miR-101 Molecular Circuit Functions as an Intrinsic Tumor-Suppressor Network EBioMedicine 7/7/2018 [PMID: 30049386]
GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth Mol. Cancer Ther., 2018-04-25;0(0):. 2018-04-25 [PMID: 29695633] (Western Blot, Human)	Western Blot	Human
N Ohoka, Y Morita, K Nagai, K Shimokawa, O Ujikawa, I Fujimori, M Ito, Y Hayase, K Okuhira, N Shibata, T Hattori, T Sameshima, O Sano, R Koyama, Y Imaeda, H Nara, N Cho, M Naito Derivatization of inhibitor of apoptosis protein (IAP) ligands yields improved inducers of estrogen receptor ? degradation J. Biol. Chem., 2018-03-15;0(0):. 2018-03-15 [PMID: 29545311] (Western Blot, Human)	Western Blot	Human
A Mohr, L Deedigan, S Jencz, Y Mehrabadi, L Houlden, SM Albarenque, RM Zwacka Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment Cell Death Differ., 2017-11-03;0(0):. 2017-11-03 [PMID: 29099485] (Western Blot, Human)	Western Blot	Human
H Takaki, Y Akazawa, Y Kido, M Morishita, T Honda, H Shibata, S Miuma, H Miyaaki, N Taura, H Kondo, K Nakao Hepatitis C Virus Infection Increases c-Jun N-Terminal Kinase (JNK) Phosphorylation and Accentuates Hepatocyte Lipoapoptosis Med. Sci. Monit., 2017-09-21;23(0):4526-4532. 2017-09-21 [PMID: 28931802] (Western Blot, Human)	Western Blot	Human
V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma Cancer Med, 2017-08-10;0(0):. 2017-08-10 [PMID: 28799237] (Western Blot, Human)	Western Blot	Human
N Shibata, N Miyamoto, K Nagai, K Shimokawa, T Sameshima, N Ohoka, T Hattori, Y Imaeda, H Nara, N Cho, M Naito Development of protein degradation inducers of oncogenic BCR-ABL protein by conjugation of ABL kinase inhibitors and IAP ligands Cancer Sci., 2017-06-19;108(8):1657-1666. 2017-06-19 [PMID: 28556300] (Western Blot, Human)	Western Blot	Human
V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues Cell Death Dis, 2017-05-25;8(5):e2816. 2017-05-25 [PMID: 28542143] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
S Biswas, JL Shisler Molluscum Contagiosum Virus MC159 abrogates cIAP1-NEMO interactions and inhibits NEMO polyubiquitination J. Virol., 2017-07-12;0(0):. 2017-07-12 [PMID: 28515292] (Western Blot, Human)	Western Blot	Human
L Zhao, G Yang, H Bai, M Zhang, D Mou NCTD promotes Birinapant-mediated anticancer activity in breast cancer cells by downregulation of c-FLIP Oncotarget, 2017-04-18;8(16):26886-26895. 2017-04-18 [PMID: 28460471] (Western Blot, Human)	Western Blot	Human
Nobumichi Ohoka, Katsunori Nagai, Norihito Shibata, Takayuki Hattori, Hiroshi Nara, Nobuo Cho et al. SNIPER(TACC3) induces cytoplasmic vacuolization and sensitizes cancer cells to Bortezomib Cancer Science 5/1/2017 [PMID: 28192613]
L Hernandez, MK Kim, AM Noonan, E Sagher, H Kohlhammer, G Wright, LT Lyle, PS Steeg, M Anver, DD Bowtell, CM Annunziata A dual role for Caspase8 and NF-?B interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival Cell Death Discov, 2015-12-14;1(0):15053. 2015-12-14 [PMID: 28179987] (Western Blot, Human)	Western Blot	Human
ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice Cell Death Dis, 2017-01-05;8(1):e2535. 2017-01-05 [PMID: 28055006] (IHC, Western Blot, Human, Mouse)	IHC, Western Blot	Human, Mouse
Keiichiro Okuhira Targeted degradation of proteins localized in subcellular compartments by hybrid small molecules Mol. Pharmacol, 2016-12-13;0(0):. 2016-12-13 [PMID: 27965304] (Western Blot, Human)	Western Blot	Human
Tchoghandjian A, Souberan A, Tabouret E et al. Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152 Cell Death Dis 8/5/2016 [PMID: 27490930]
Katrin E. Tagscherer, Anne Fassl, Tabea Sinkovic, Jutta Richter, Sabrina Schecher, Stephan Macher-Goeppinger et al. MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen Cancer Cell International 12/1/2016 [PMID: 27293381]
Simon PS, Bardhan K, Chen MR et al. NF-kB functions as a molecular link between tumor cells and Th1/Tc1 T cells in the tumor microenvironment to exert radiation-mediated tumor suppression. Oncotarget. 4/26/2016 [PMID: 27014915]
M Schirmer, L Trentin, M Queudeville, F Seyfried, S Demir, E Tausch et al. Intrinsic and chemo-sensitizing activity of SMAC-mimetics on high-risk childhood acute lymphoblastic leukemia Cell Death & Disease 1/14/2016 [PMID: 26775704]
Metzig MO, Fuchs D, Tagscherer KE, Grone HJ. Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-a-dependent necroptosis driven by RIP1 kinase and NF-kB. Oncogene. 11/2/2015 [PMID: 26522725]
Hehlgans S, Oppermann J, Reichert S et al. The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis. Radiat Oncol 9/19/2015 [PMID: 26383618]
Lee E, Seong D, Seo J, Jeong M, Lee H, Song J USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics. Cell Death Differ, 2015-01-23;22(9):1463-76. 2015-01-23 [PMID: 25613375] (Western Blot, Human)	Western Blot	Human
Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs). Anticancer Res, 2014-12-01;34(12):7007-20. 2014-12-01 [PMID: 25503127] (Western Blot, Human)	Western Blot	Human
Schoeneberger H, Belz K, Schenk B, Fulda S Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death. Oncogene, 2014-11-10;34(31):4032-43. 2014-11-10 [PMID: 25381820] (Western Blot, Human)	Western Blot	Human
N Ohoka, K Nagai, T Hattori, K Okuhira, N Shibata, N Cho et al. Cancer cell death induced by novel small molecules degrading the TACC3 protein via the ubiquitin–proteasome pathway Cell Death & Disease 11/6/2014 [PMID: 25375378]
Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J, Zhang X, Dai S, Sun X Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair. J Hematol Oncol, 2014-08-20;7(0):62. 2014-08-20 [PMID: 25139395] (Western Blot, Human)	Western Blot	Human
Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. Int J Oncol, 2014-04-28;45(1):419-25. 2014-04-28 [PMID: 24789756] (Flow Cytometry, Human)	Flow Cytometry	Human
Guicciardi , Maria Eu, Werneburg , Nathan W, Bronk , Steven F, Franke , Adrian, Yagita , Hideo, Thomas , Gary, Gores , Gregory Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity. PLoS ONE, 2014-03-14;9(3):e92124. 2014-03-14 [PMID: 24633224] (Western Blot, Human)	Western Blot	Human
Uno M, Saitoh Y, Mochida K, Tsuruyama E, Kiyono T, Imoto I, Inazawa J, Yuasa Y, Kubota T, Yamaoka S NF-kappaB inducing kinase, a central signaling component of the non-canonical pathway of NF-kappaB, contributes to ovarian cancer progression. PLoS ONE, 2014-02-12;9(2):e88347. 2014-02-12 [PMID: 24533079] (Western Blot, Human)	Western Blot	Human
Wang Q, Chen W, Bai L, Chen W, Padilla M, Lin A, Shi S, Wang X, Lin Y Receptor-interacting protein 1 increases chemoresistance by maintaining inhibitor of apoptosis protein levels and reducing reactive oxygen species through a microRNA-146a-mediated catalase pathway. J Biol Chem, 2014-01-14;289(9):5654-63. 2014-01-14 [PMID: 24425875] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. FASEB J, 2013-12-26;28(4):1938-46. 2013-12-26 [PMID: 24371121] (Western Blot, Human)	Western Blot	Human
Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells. Clin Cancer Res, 2013-02-12;19(7):1784-94. 2013-02-12 [PMID: 23403634] (Western Blot, Human)	Western Blot	Human
Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma. PLoS ONE, 2012-09-20;7(9):e45492. 2012-09-20 [PMID: 23029050] (Western Blot, Human)	Western Blot	Human
Varfolomeev , Eugene, Moradi , Elham, Dynek , Jasmin N, Zha , Jiping, Fedorova , Anna V, Deshayes , Kurt, Fairbrother , Wayne J, Newton , Kim, Le Couter , Jennifer, Vucic , Domagoj Characterization of ML-IAP protein stability and physiological role in vivo. Biochem J, 2012-11-01;447(3):427-36. 2012-11-01 [PMID: 22853455] (Western Blot, Human)	Western Blot	Human
Oberoi-Khanuja , Tripat K, Karreman , Christia, Larisch , Sarit, Rapp , Ulf R, Rajalingam , Krishnar Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration. J Biol Chem, 2012-06-18;287(34):28445-55. 2012-06-18 [PMID: 22711539] (Western Blot, Human)	Western Blot	Human
Role of Apollon in human melanoma resistance to antitumor agents that activate the intrinsic or the extrinsic apoptosis pathways. Clin. Cancer Res., 2012-05-02;18(12):3316-27. 2012-05-02 [PMID: 22553342] (Flow Cytometry, Western Blot, Human)	Flow Cytometry, Western Blot	Human
Wagner L, Marschall V, Karl S, Cristofanon S, Zobel K, Deshayes K, Vucic D, Debatin K, Fulda S Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-kappaB-dependent manner. Oncogene, 2012-04-02;32(8):988-97. 2012-04-02 [PMID: 22469979] (Western Blot, Human)	Western Blot	Human
Hendrik J. Kuiken, David A. Egan, Heike Laman, Rene Bernards, Roderick L. Beijersbergen, Annette M. Dirac Identification of F-box only protein 7 as a negative regulator of NF-kappaB signalling Journal of Cellular and Molecular Medicine 9/1/2012 [PMID: 22212761]
Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis. Leukemia, 2011-12-16;26(5):1020-9. 2011-12-16 [PMID: 22173242] (Western Blot, Human)	Western Blot	Human
Ikner A, Ashkenazi A TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8. J. Biol. Chem., 2011-04-27;286(24):21546-54. 2011-04-27 [PMID: 21525013] (Western Blot, Human)	Western Blot	Human
Isabelle Coupienne, Sébastien Bontems, Michael Dewaele, Noemi Rubio, Yvette Habraken, Simone Fulda et al. NF-kappaB inhibition improves the sensitivity of human glioblastoma cells to 5-aminolevulinic acid-based photodynamic therapy Biochemical Pharmacology 3/1/2011 [PMID: 21182827]
Adina Stanculescu, Leslie A. Bembinster, Kristina Borgen, Anna Bergamaschi, Elizabeth Wiley, Jonna Frasor Estrogen Promotes Breast Cancer Cell Survival in an Inhibitor of Apoptosis (IAP)-dependent Manner Hormones and Cancer 12/15/2010 [PMID: 21152357]
Guicciardi ME, Mott JL, Bronk SF et al. Cellular Inhibitor of Apoptosis 1 (cIAP-1) Degradation by Caspase 8 During TNF-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis. Exp Cell Res. 10/13/2010 [PMID: 20951133]
Reddy RM, Yeow WS, Chua A, Nguyen DM, Baras A, Ziauddin MF, Shamimi-Noori SM, Maxhimer JB, Schrump DS Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade. Apoptosis, 2007-01-01;12(1):55-71. 2007-01-01 [PMID: 17136498] (Western Blot, Human)	Western Blot	Human
Balasubramanian S, Mani S, Shiraishi H, Johnston RK, Yamane K, Willey CD, Cooper G, Tuxworth WJ, Kuppuswamy D Enhanced ubiquitination of cytoskeletal proteins in pressure overloaded myocardium is accompanied by changes in specific E3 ligases. J. Mol. Cell. Cardiol., 2006-08-22;41(4):669-79. 2006-08-22 [PMID: 16928382] (Western Blot, Feline)	Western Blot	Feline
Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC cIAP1 Localizes to the nuclear compartment and modulates the cell cycle. Cancer Res., 2005-01-01;65(1):210-8. 2005-01-01 [PMID: 15665297] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications. Oncogene, 2002-08-22;21(37):5673-83. 2002-08-22 [PMID: 12173037] (Western Blot, Human)	Western Blot	Human
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Reviews for cIAP-1/HIAP-2 Antibody (AF8181) (4) 44

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01/09/2015

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Application	Western Blot
Sample Tested	See PMID 22553342
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01/09/2015

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Application	Western Blot
Sample Tested	See PMID 22212761
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Application	Western Blot
Sample Tested	See PMID 20074295
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01/09/2015

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Application	Western Blot
Sample Tested	See PMID 22711539
Species	Human

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cIAP1 - An apoptotic regulator with implications in drug resistant cancers
Cellular inhibitor of apoptosis protein-1 (cIAP-1) is an anti-apoptotic protein that is able to bind to caspases and inhibit their activity. Additionally cIAP-1 contains a RING domain with E3 ubiquitin ligase activity that is able to mediate the r... Read full blog post.

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Goat IgG Isotype Control [Unconjugated]
AB-108-C

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

TNF-alpha [Unconjugated]
210-TA-005

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Application:	WB
Species:	Human

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	Verified Customer 01/09/2015
Application:	WB
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	Verified Customer 01/09/2015
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Species:	Human

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Bioinformatics

Gene Symbol	BIRC2
Uniprot	Q13490()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

cIAP-1/HIAP-2 Antibody [Unconjugated]

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Publications for cIAP-1/HIAP-2 Antibody (AF8181)(66)

Reviews for cIAP-1/HIAP-2 Antibody (AF8181) (4) 44

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