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CEBP alpha Antibody (C9)

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Immunocytochemistry /Immunofluorescence: CEBP alpha Antibody (C9) [NBP3-41783] - FITC staining on IF; Sample: Human Hela cell; Primary Ab: 30ug/ml NBP3-41783; Second Ab: 2ug/ml FITC-Linked Caprine Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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CEBP alpha Antibody (C9) Summary

Immunogen
Immunized with recombinant Rat CEBP alpha (Uniprot#: P05554; Ala72-Ala358 (Uniprot#: P05554)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEBPA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5-20ug/mL
  • Immunohistochemistry 5-20ug/mL
  • Western Blot 0.5-2ug/mL

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01M PBS, pH7.4, 50% glycerol
Preservative
0.05% Proclin 300
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CEBP alpha Antibody (C9)

  • C/EBP alpha
  • C/EBP-alpha
  • CCAAT/enhancer binding protein (C/EBP), alpha
  • CCAAT/enhancer-binding protein alpha
  • CEBP alpha
  • CEBP
  • CEBPA

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CEBP alpha Antibody (NBP3-41783) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CEBP alpha Antibody (NBP3-41783)

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Blogs on CEBP alpha.

Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

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Bioinformatics

Gene Symbol CEBPA