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CD47 Antibody (CD47/3019)

Images

 
Immunocytochemistry/ Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975] - Immunofluorescence staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Biotin-conjugated CD47 Mouse Monoclonal Antibody (IAP/3019).
Flow Cytometry: CD47 Antibody (CD47/3019) [NBP3-08975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (blue); isotype control ...read more
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Mouse Monoclonal Antibody (CD47/3019).
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Mouse Monoclonal Antibody (CD47/3019).
Protein Array: CD47 Antibody (CD47/3019) [NBP3-08975] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/3019).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, MA
Clone
CD47/3019
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD47 Antibody (CD47/3019) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08976)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary) (Uniprot: Q08722)
Localization
Cell Surface
Specificity
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD47
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
ELISA: For coating, order without BSA
Optimal dilution for a specific application should be determined.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD47 Antibody (CD47/3019)

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Background

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD47
Uniprot