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CD47 Antibody (CD47/3019) [PE]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, MA
Clone
CD47/3019
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

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CD47 Antibody (CD47/3019) [PE] Summary

Immunogen
A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary) (Uniprot: Q08722)
Localization
Cell Surface
Specificity
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD47
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD47 Antibody (CD47/3019) [PE]

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Background

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD47