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CD47 Antibody (CD47/3019) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, MA
Clone
CD47/3019
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08976AF350
Alexa Fluor 405
NBP3-08976AF405
Alexa Fluor 488
NBP3-08976AF488
Alexa Fluor 532
NBP3-08976AF532
Alexa Fluor 594
NBP3-08976AF594
Alexa Fluor 647
NBP3-08976AF647
Alexa Fluor 700
NBP3-08976AF700
Alexa Fluor 750
NBP3-08976AF750
Allophycocyanin
NBP3-08976APC
Biotin
NBP3-08976B
DyLight 350
NBP3-08976UV
DyLight 405
NBP3-08976V
DyLight 488
NBP3-08976G
DyLight 550
NBP3-08976R
DyLight 594
NBP3-08976DL594
DyLight 650
NBP3-08976C
DyLight 680
NBP3-08976FR
DyLight 755
NBP3-08976IR
FITC
NBP3-08976F
HRP
NBP3-08976H
Janelia Fluor 525
NBP3-08976JF525
Janelia Fluor 549
NBP3-08976JF549
Janelia Fluor 646
NBP3-08976JF646
Janelia Fluor 669
NBP3-08976JF669
PE
NBP3-08976PE
PerCP
NBP3-08976PCP
Unconjugated
NBP3-08975-100ug
mFluor Violet 450 SE
NBP3-08976MFV450
mFluor Violet 500 SE
NBP3-08976MFV500
mFluor Violet 610 SE
NBP3-08976MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-08975-100ug
Azide and BSA Free
NBP3-08976
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CD47 Antibody (CD47/3019) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary) (Uniprot: Q08722)
Localization
Cell Surface
Specificity
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD47
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CD47 Antibody (CD47/3019) [CoraFluor™ 1]

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Background

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD47