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CD44 Antibody (OX-50) - Azide and BSA Free

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Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaini
Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was ...read more
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OX-50
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-1317AF350
Alexa Fluor 405
NB600-1317AF405
Alexa Fluor 488
NB600-1317AF488
Alexa Fluor 532
NB600-1317AF532
Alexa Fluor 594
NB600-1317AF594
Alexa Fluor 647
NB600-1317AF647
Alexa Fluor 700
NB600-1317AF700
Alexa Fluor 750
NB600-1317AF750
Allophycocyanin
NB600-1317APC
Allophycocyanin/Cy7
NB600-1317APCCY7
Biotin
NB600-1317B
CoraFluor 1
NB600-1317CL1
DyLight 350
NB600-1317UV
DyLight 405
NB600-1317V
DyLight 488
NB600-1317G
DyLight 550
NB600-1317R
DyLight 594
NB600-1317DL594
DyLight 650
NB600-1317C
DyLight 680
NB600-1317FR
DyLight 755
NB600-1317IR
FITC
NB600-1317F
HRP
NB600-1317H
Janelia Fluor 525
NB600-1317JF525
Janelia Fluor 549
NB600-1317JF549
Janelia Fluor 646
NB600-1317JF646
Janelia Fluor 669
NB600-1317JF669
PE
NB600-1317PE
PE/Atto594
NB600-1317PEATT594
PE/Cy5.5
NB600-1317PECY55
PE/Cy7
NB600-1317PECY7
PerCP
NB600-1317PCP
Unconjugated
NB600-1317
mFluor Violet 450 SE
NB600-1317MFV450
mFluor Violet 500 SE
NB600-1317MFV500
mFluor Violet 610 SE
NB600-1317MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB600-1317
BSA Free
NB600-1317
BSA Free
NB600-1317SS
Novus Biologicals offers 100% Guarantee

CD44 Antibody (OX-50) - Azide and BSA Free Summary

Immunogen
Rat T cell blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.
Localization
Type I membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:50 - 1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Frozen 1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
  • Immunohistochemistry-Paraffin 1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
  • Western Blot reported in scientific literature (PMID 8537452)
Application Notes
This antibody is CyTOF ready.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD44 Antibody (OX-50) - Azide and BSA Free

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44 is a glycoprotrein of 85 kDa, with expression being increased following activation. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol CD44