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CD44 Antibody (8E2F3) - BSA Free

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Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye ...read more
Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were ...read more
Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Western blot analysis of ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA ...read more
Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either ...read more
Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. d) Western blot analysis of pSTAT3, STAT3, and CD44 in primary keratinocytes and IPKs from ...read more
CD44 (8E2F3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Biotin (Catalog # NBP1-47386B) at 2 µg/mL overnight ...read more
CD44 (8E2F3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-47386R) (red) at 5 µg/mL ...read more
U-251 MG human glioblastoma cell line was stained with Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-47386AF647, blue histogram) or matched control ...read more

Product Details

Summary
Reactivity Hu, Mu, Rb(-), Pm, Ca, EqSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clone
8E2F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD44 Antibody (8E2F3) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD44 (628-699) expressed in E. coli. [Uniprot: P16070]
Localization
Membrane; Single-pass type I membrane protein.
Marker
Cell Membrane Marker
Predicted Species
Baboon (94%), Equine (93%), Canine (93%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation
  • Western Blot
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47386 in the following applications:

Publications
Read Publications using
NBP1-47386 in the following applications:

Reactivity Notes

Not reactive to rabbit per customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for CD44 Antibody (8E2F3) - BSA Free

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44 Antibody (NBP1-47386)(19)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-P, PAGE, WB.


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FLOW
(5)
ICC/IF
(2)
IF/IHC
(3)
IHC-P
(4)
PAGE
(1)
WB
(4)
All Applications
Filter By Species
Human
(7)
Mouse
(7)
Rat
(1)
Canine
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-47386 Applications Species
Lubanska D, Alrashed S, Mason GT et al. Impairing proliferation of glioblastoma multiforme with CD44+ selective conjugated polymer nanoparticles Scientific reports 2022-07-15 [PMID: 35840697] (PAGE) PAGE
Marzban H, Sasani F Canine Mammary Gland Cancer Stem Cell and its Potential Role in Malignant Biologic Behavior Iranian Journal of Veterinary Medicine 2020-01-01 (WB, Canine) WB Canine
Saxena N, Bhardwaj G, Jadhav S, et al. Pre-metastatic niche drives breast cancer invasion by modulating MSC homing and CAF differentiation bioRxiv 2021-01-15 (ICC/IF, Human) ICC/IF Human
Salman S, Meyers DJ, Wicks EE Et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 2022-05-02 [PMID: 35499076] (FLOW, Mouse)

Details:
Citation using the Alexa Fluor 488 version of this antibody.
FLOW Mouse
Li H, Chaitankar V, Zhu J et al. Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC Scientific reports 2020-12-14 [PMID: 33318499] (ICC/IF, IHC-P, Human) ICC/IF, IHC-P Human
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 2020-04-02 [PMID: 32243809] (Mouse) Mouse
Scimeca M, Urbano N, Bonfiglio R et al. Breast osteoblast-like cells: a new biomarker for the management of breast cancer. Br. J. Cancer. 2018-10-17 [PMID: 30327566] (IHC-P, Human) IHC-P Human
Hassanzadeh H, Matin MM, Naderi-Meshkin H et al. Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications Cell Tissue Bank 2018-08-13 [PMID: 30105667] (FLOW, Human) FLOW Human
Baek M, kim M, Lim JS et al. Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNk signaling. Cell Death Dis. 2018-06-28 [PMID: 29955047] (WB, Mouse) WB Mouse
Jeong YJ, Oh HK, Park SH, Bong JG. Association between inflammation and cancer stem cell phenotype in breast cancer. Nat Commun. [PMID: 29434947] (IHC-P, Human) IHC-P Human
Show All 19 Publications.

Review for CD44 Antibody (NBP1-47386) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-47386:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CD44 NBP1-47386
Enlarge
5
reviewed by:
Corey .
WB Human 07/12/2013
View

Summary

ApplicationWestern Blot
Sample TestedHuman Brain Tissue
SpeciesHuman
Lot130226

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD44 Antibody (NBP1-47386) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CD44 Products

Research Areas for CD44 Antibody (NBP1-47386)

Find related products by research area.

Blogs on CD44.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The Osteopontin Antibody and Hepatic Research
Antibody suppliers, such as us at Novus Biologicals, supply a wide range of cell marker products, among them the Osteopontin antibody. Recently, the osteopontin antibody has proven useful in hepatic cancer research.Osteopontin (OPN) is mainly expres...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Corey .
07/12/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol CD44
Entrez
Uniprot