Home » CD40/TNFRSF5 » CD40/TNFRSF5 Antibodies » CD40/TNFRSF5 Antibody (65924) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CD40/TNFRSF5 Antibody (65924) [Unconjugated]

 (10 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for CD40/TNFRSF5 Antibody (MAB4401).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap)
Clone
65924
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4401U-100UG
Alexa Fluor 405
FAB4401V-100UG
Alexa Fluor 488
FAB4401G-100UG
Alexa Fluor 532
FAB4401X
Alexa Fluor 594
FAB4401T-100UG
Alexa Fluor 647
FAB4401R-100UG
Alexa Fluor 700
FAB4401N-100UG
Alexa Fluor 750
FAB4401S-100UG
Allophycocyanin
FAB4401A
Allophycocyanin/Cy7
FAB4401APCCY7
Biotin
FAB4401B
DyLight 350
FAB4401D
DyLight 405
FAB4401E
DyLight 488
FAB4401K
DyLight 550
FAB4401L
DyLight 594
FAB4401M
DyLight 650
FAB4401W
DyLight 680
FAB4401Y
DyLight 755
FAB4401Z
FITC
FAB4401F
HRP
FAB4401H
Janelia Fluor 525
FAB4401JF525
Janelia Fluor 549
FAB4401I
Janelia Fluor 585
FAB4401JF585
Janelia Fluor 635
FAB4401JF635
Janelia Fluor 646
FAB4401J
Janelia Fluor 669
FAB4401JF669
PE/Atto594
FAB4401PEATT594
PE/Cy5.5
FAB4401PECY55
PE/Cy7
FAB4401PECY7
PerCP
FAB4401C
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4401-SP
Carrier Free
FAB4401V-100UG
Carrier Free
FAB4401U-100UG
Carrier Free
FAB4401T-100UG
Carrier Free
FAB4401S-100UG
Carrier Free
FAB4401R-100UG
Carrier Free
FAB4401N-100UG
Carrier Free
FAB4401G-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CD40/TNFRSF5 Antibody (65924) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD40/TNFRSF5
Val24-Arg193
Accession # P27512
Specificity
Detects mouse CD40/TNFRSF5 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CD40/TNFRSF5, recombinant mouse (rm) CD40 Ligand, rmCD27/TNFRSF7, rmCD30/TNFRSF8, or rmFas/TNFRSF6 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CD40
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Mouse CD40/TNFRSF5 Biotinylated Antibody (Catalog number BAF440)
Standard: Recombinant Mouse CD40/TNFRSF5 Fc Chimera (Catalog number 1215-CD)
Publications
Read Publications using
MAB4401 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD40/TNFRSF5 Antibody (65924) [Unconjugated]

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Background

CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).

  1. Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
  2. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  3. Armitage, R.J. et al. (1993) J. Immunol. 150:3671.
  4. Callard, R.E. et al. (1993) Immunol. Today 14:559.
  5. Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487.
  6. Pullen, S.S. et al. (1999) Biochemistry 38:10168.
  7. Faris, M. et al. (1994) J. Exp. Med. 179:1923.
  8. Hanissian, S.H. and R.S. Geha (1997) Immunity 6:379.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CD40 Ligand/TNFSF5
[HRP]
DCDL40
N/A CD40 Ligand/TNFSF5 [HRP]
N/A CD40 Ligand/TNFSF5 [HRP]
(44 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(810 Publications)
Species: Hu
Applications: BA
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
B7-2/CD86 Antibody
(BU63)
NBP2-25208
Knockout Validated B7-2/CD86 Antibody (BU63)
Immunocytochemistry/ Immunofluorescence B7-2/CD86 Antibody (BU63)
Flow Cytometry B7-2/CD86 Antibody (BU63)
(27 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
B7-1/CD80 Antibody
(37711) [Unconjugated ...
MAB140
Flow Cytometry B7-1/CD80 Antibody (37711) [Unconjugated]
Immunohistochemistry B7-1/CD80 Antibody (37711) [Unconjugated]
Dual RNAscope ISH-IHC Compatible B7-1/CD80 Antibody (37711) [Unconjugated]
(26 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
Human, Mouse, Rat
RelA/NFkB p65 ELISA K ...
NBP2-29661
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
(95 Publications)
Species: Hu, Mu, Rt
Applications: ELISA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Synaptotagmin 1
Antibody (ASV48) [Unc ...
MAB4364
Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
Immunohistochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
(1 Publication)
Species: Rt
Applications: ICC, IHC, IP, KO, WB
WNK1 Antibody
[Unconjugated]
AF2849
Western Blot WNK1 Antibody [Unconjugated]
(5 Publications)
Species: Mu, Rt
Applications: WB
GRASP65 Antibody
(OTI5G8)
NBP2-02665
Immunocytochemistry/ Immunofluorescence GRASP65 Antibody (OTI5G8)
Immunocytochemistry/ Immunofluorescence GRASP65 Antibody (OTI5G8)
Flow Cytometry GRASP65 Antibody (OTI5G8)
(3 Reviews)
(2 Publications)
Species: Ca, Hu, Pm, Po, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(299 Publications)
Species: Mu
Applications: ELISA
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(650 Publications)
Species: Mu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(450 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
ICAM-1/CD54
Antibody [Unconjugate
AF796
Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
(6 Reviews)
(88 Publications)
Species: Mu
Applications: AdBlk, IHC, WB

Publications for CD40/TNFRSF5 Antibody (MAB4401)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Luminex Development.


Filter By Application
Luminex Development
(1)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB4401 Applications Species
I Fagerlund, A Dougalis, A Shakirzyan, M Gómez-Budi, A Pelkonen, H Konttinen, S Ohtonen, MF Fazaludeen, M Koskuvi, J Kuusisto, D Hernández, A Pebay, J Koistinaho, T Rauramaa, Š Lehtonen, P Korhonen, T Malm Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids Cells, 2021-12-30;11(1):. 12/30/2021 [PMID: 35011686]
J Tiihonen, M Koskuvi, M Storvik, I Hyötyläine, Y Gao, KA Puttonen, R Giniatulli, E Poguzhelsk, I Ojansuu, O Vaurio, TD Cannon, J Lönnqvist, S Therman, J Suvisaari, J Kaprio, L Cheng, AF Hill, M Lähteenvuo, J Tohka, R Giniatulli, Š Lehtonen, J Koistinaho Sex-specific transcriptional and proteomic signatures in schizophrenia Nat Commun, 2019-09-02;10(1):3933. 9/2/2019 [PMID: 31477693]
M Hou, W Wang, F Hu, Y Zhang, D Yang, Q Liu Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner Infect. Immun., 2018-12-19;87(1):. 12/19/2018 [PMID: 30396897]
Wang Xi, Dong Ke, Long Min, Wang Lin, Zuo Jiahui, Lin Fang et al. Incorporation of CD40 ligand enhances the immunogenicity of tumor‑associated calcium signal transducer 2 virus‑like particles against lung cancer International Journal of Molecular Medicine 3/15/2018 [PMID: 29568866]
P Walsh, V Truong, C Hill, ND Stoflet, J Baden, WC Low, SA Keirstead, JR Dutton, AM Parr Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells Cell Transplant, 2017-12-01;26(12):1890-1902. 12/1/2017 [PMID: 29390875]
Paulina Carriba, Alun M Davies CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons eLife 11/7/2017 [PMID: 29111976]
CY Yu, TC Li, YY Wu, CH Yeh, W Chiang, CY Chuang, HC Kuo The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency Nat Commun, 2017-10-27;8(1):1149. 10/27/2017 [PMID: 29074849]
Li Zou, Qingshan Chen, Zachary Quanbeck, Joan E. Bechtold, Dan S. Kaufman Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells Scientific Reports 3/16/2016 [PMID: 26980556]
Nienke Roescher, Jelle L. Vosters, Zhenan Lai, Toshimitsu Uede, Paul P. Tak, John A. Chiorini Local Administration of Soluble CD40:Fc to the Salivary Glands of Non-Obese Diabetic Mice Does Not Ameliorate Autoimmune Inflammation PLoS ONE 12/26/2012 [PMID: 23300544]
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse

Reviews for CD40/TNFRSF5 Antibody (MAB4401) (0)

There are no reviews for CD40/TNFRSF5 Antibody (MAB4401). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD40/TNFRSF5 Antibody (MAB4401). (Showing 1 - 2 of 2 FAQs).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Secondary Antibodies

 

Isotype Controls

Additional CD40/TNFRSF5 Products

Blogs on CD40/TNFRSF5

There are no specific blogs for CD40/TNFRSF5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Mouse platelets were stained with Rat Anti-Mouse GPVIMonoclonal Antibody (Catalog # MAB6758,filled histogram) or Rat IgG Isotype Control Antibody (Catalog # MAB005,open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113).
Rat IgG1 Isotype Control (43414) [Unconjugated]
MAB005
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD40/TNFRSF5 Antibody (65924) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CD40
Uniprot