Detects mouse CD40/TNFRSF5 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CD40/TNFRSF5, recombinant mouse (rm) CD40 Ligand, rmCD27/TNFRSF7, rmCD30/TNFRSF8, or rmFas/TNFRSF6 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CD40
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CD40/TNFRSF5 Antibody (65924) [Unconjugated]
TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
tumor necrosis factor receptor superfamily member 5
tumor necrosis factor receptor superfamily, member 5
Background
CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).
Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
Armitage, R.J. et al. (1993) J. Immunol. 150:3671.
Callard, R.E. et al. (1993) Immunol. Today 14:559.
Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487.
Pullen, S.S. et al. (1999) Biochemistry 38:10168.
Faris, M. et al. (1994) J. Exp. Med. 179:1923.
Hanissian, S.H. and R.S. Geha (1997) Immunity 6:379.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CD40/TNFRSF5 Antibody (MAB4401). (Showing 1 - 2 of 2 FAQs).
I wanna know does CD40 antibody for IHC-P comes with positive control slide?
Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
Unfortunately, none of our CD40 antibodies have been specifically tested for this application.
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